Sullivan Asa, Nord Carl Erik
From the Division of Clinical Bacteriology, Karolinska University Hospital Huddinge, Karolinska Institute, Stockholm, Sweden.
Scand J Infect Dis. 2006;38(5):327-31. doi: 10.1080/00365540500449826.
The objective of the present study was to determine the incidence of lactobacilli-induced bacteraemia in Stockholm, Sweden, between January 1998 and March 2004, and to identify the possible presence of probiotic strains. Isolated strains were checked for growth on selective lactobacilli-agar and the biochemical profiles were determined. The lactobacilli strains were further analysed with molecular methodologies to ascertain eventual similarities with the probiotic strains Lactobacillus paracasei subsp. paracasei F19 (LMG P 17806), Lactobacillus acidophilus NCFB 1748 and Lactobacillus rhamnosus GG (ATCC 53103). The minimum inhibitory concentrations of selected antimicrobial agents were determined by the agar dilution method. The incidence of bacteraemia cases caused by lactobacilli remained at the same level during the study period and constituted to <1% of the total number of bacteraemia cases each y. L. paracasei subsp. paracasei F19, L. acidophilus NCFB 1748 and L. rhamnosus GG were not identified in any of the samples.
本研究的目的是确定1998年1月至2004年3月期间瑞典斯德哥尔摩乳杆菌引起的菌血症发病率，并确定是否可能存在益生菌菌株。检查分离菌株在选择性乳杆菌琼脂上的生长情况，并测定其生化特征。进一步采用分子方法对乳杆菌菌株进行分析，以确定其与益生菌副干酪乳杆菌副干酪亚种F19（LMG P 17806）、嗜酸乳杆菌NCFB 1748和鼠李糖乳杆菌GG（ATCC 53103）是否存在相似性。采用琼脂稀释法测定所选抗菌药物的最低抑菌浓度。在研究期间，乳杆菌引起的菌血症病例发病率保持在同一水平，每年占菌血症病例总数的比例不到1%。在任何样本中均未鉴定出副干酪乳杆菌副干酪亚种F19、嗜酸乳杆菌NCFB 1748和鼠李糖乳杆菌GG。
