病毒转化的人细胞产生但不保留成纤维细胞表面抗原。
Fibroblast surface antigen produced but not retained by virus-transformed human cells.
作者信息
Vaheri A, Ruoslahti E
出版信息
J Exp Med. 1975 Aug 1;142(2):530-5. doi: 10.1084/jem.142.2.530.
Abstract
Normal human fibroblasts contain a cell type-specific glycoprotein antigen (SF) that is known to be slowly shed into the medium and to be present also in human serum. Immunofluorescence with anti-SF antibodies showed that SF antigen has a highly nonrandom fibrillar distribution in surface of normal fibroblasts. Simian virus 40-transformed fibroblasts also produced the SF antigen, as shown by radioimmunoassay or immunodiffusion tests, but it was not retained by the surface of these cells. This creates a major difference between the surfaces of normal and malignant cells.
摘要
正常人类成纤维细胞含有一种细胞类型特异性糖蛋白抗原（SF），已知其会缓慢释放到培养基中，并且也存在于人类血清中。用抗SF抗体进行免疫荧光检测显示，SF抗原在正常成纤维细胞表面具有高度非随机的纤维状分布。如放射免疫测定或免疫扩散试验所示，猿猴病毒40转化的成纤维细胞也产生SF抗原，但这些细胞的表面并不保留该抗原。这在正常细胞和恶性细胞的表面之间造成了重大差异。
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