Bacci B, Morandi F, De Meo M, Marcato P S
Dipartimento di Sanità Pubblica Veterinaria e Patologia Animale, Alma Mater Studiorum Università di Bologna, Via Tolara di Sopra 50, Ozzano Emilia, 40064 Bologna, Italy.
J Comp Pathol. 2006 May;134(4):347-54. doi: 10.1016/j.jcpa.2006.02.001. Epub 2006 May 18.
In the cat only 10 cases of mesothelioma, mainly of the peritoneum, have been previously reported. This paper describes a further 10 cases, eight pleural and two peritoneal, in males and females aged 1-17 years. Histologically, five tumours were epithelial, three fibrosarcomatous and two biphasic. Immunohistochemical markers used in human pathology for the identification of mesotheliomas include vimentin, cytokeratin (CK) AE1/AE3, HBME-1, CK 5/6, calretinin, thrombomodulin, carcinoembryonic antigen (CEA), CD15, E-cadherin and desmin. All 10 feline mesotheliomas were positive for vimentin and CK AE1/AE3, six were positive for HBME-1, two for CK5/6, three for CEA and four for E-cadherin. All were negative for desmin and calretinin. Antibodies to thrombomodulin and CD15 failed to cross-react with feline tissues. Electron microscopy, performed in four cases, revealed microvillar structures, desmosomes and intracytoplasmic lumina, confirming its value as a diagnostic tool. The study showed that mesothelial marker antibodies commonly used in human patients can be used for the diagnosis of feline mesothelioma, preferably as a panel of antibodies rather than only one.
此前仅报道过10例猫的间皮瘤病例，主要发生于腹膜。本文描述了另外10例病例，其中8例为胸膜间皮瘤，2例为腹膜间皮瘤，患者年龄在1至17岁之间，有男有女。组织学上，5例肿瘤为上皮型，3例为纤维肉瘤型，2例为双向型。人类病理学中用于鉴定间皮瘤的免疫组织化学标志物包括波形蛋白、细胞角蛋白（CK）AE1/AE3、HBME-1、CK 5/6、钙网膜蛋白、血栓调节蛋白、癌胚抗原（CEA）、CD15、E-钙黏蛋白和结蛋白。所有10例猫间皮瘤波形蛋白和CK AE1/AE3均呈阳性，6例HBME-1呈阳性，2例CK5/6呈阳性，3例CEA呈阳性，4例E-钙黏蛋白呈阳性。所有病例结蛋白和钙网膜蛋白均为阴性。血栓调节蛋白和CD15抗体未能与猫组织发生交叉反应。对4例病例进行了电子显微镜检查，发现有微绒毛结构、桥粒和胞质内管腔，证实了其作为诊断工具的价值。该研究表明，人类患者常用的间皮标志物抗体可用于猫间皮瘤的诊断，最好作为一组抗体而非仅一种抗体使用。
