以伪狂犬病病毒扩增子为载体，比较即刻早期巨细胞病毒（IE-CMV）启动子和gG伪狂犬病病毒（gG-PRV）启动子诱导的增强型绿色荧光蛋白表达。

A comparison of enhanced green fluorescent protein expression induced by immediate-early cytomegalovirus (IE-CMV) and gG pseudorabies virus (gG-PRV) promoters, using pseudorabies virus amplicons as vectors.

作者信息

Muñoz A L, Prieto C, Tabarés E

机构信息

Departamento de Medicina Preventiva, Salud Pública y Microbiología, Facultad de Medicina, Universidad Autónoma de Madrid, Arzobispo Morcillo 4, E-28029 Madrid, Spain.

出版信息

J Virol Methods. 2006 Sep;136(1-2):257-60. doi: 10.1016/j.jviromet.2006.04.010. Epub 2006 May 19.

DOI:10.1016/j.jviromet.2006.04.010

PMID:16712964

Abstract

This study compares the expression efficiencies of the IE-CMV and gG-PRV promoters following their transfection into cultured human and monkey cells, using pseudorabies virus amplicons as vectors and enhanced green fluorescence protein (EGFP) as an expression marker. EGFP expression was similarly strong with both promoters. Pseudorabies virus amplicons appear to be useful vectors in gene expression studies due to their replication in the presence of helpers and their wide range of cellular hosts.

摘要

本研究以伪狂犬病病毒扩增子为载体、增强型绿色荧光蛋白（EGFP）为表达标记，比较了IE-CMV启动子和gG-PRV启动子转染培养的人细胞和猴细胞后的表达效率。两种启动子的EGFP表达均同样强烈。伪狂犬病病毒扩增子因其在辅助病毒存在下可复制且细胞宿主范围广泛，似乎是基因表达研究中的有用载体。