一种抑制立即早期基因转录的嵌合转基因产物对伪狂犬病病毒复制的抑制作用。
Inhibition of pseudorabies virus replication by a chimeric trans-gene product repressing transcription of the immediate-early gene.
作者信息
Ono E, Sakoda Y, Taharaguchi S, Watanabe S, Tonomura N, Kida H, Shimizu Y
机构信息
Department of Hygiene and Microbiology, School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
出版信息
Virology. 1995 Jun 20;210(1):128-40. doi: 10.1006/viro.1995.1324.
A chimeric gene encoding a fusion protein consisting of the DNA-binding domain of the immediate-early (IE) protein of pseudorabies virus (PRV) and a tail-truncated Vmw65 of herpes simplex virus 1, lacking the transcription activation domain, was constructed. The chimeric gene product inhibited transcription from the PRV IE promoter in a transient expression assay. A HeLa cell line stably transformed with the chimeric gene showed remarkable resistance to PRV infection. In the transformed cells infected with PRV, transcription of the PRV IE gene was repressed, indicating that the resistance of the cells to PRV infection was due to interference with IE gene transcription by the fusion protein.
构建了一个嵌合基因，其编码一种融合蛋白，该融合蛋白由伪狂犬病病毒（PRV）立即早期（IE）蛋白的DNA结合结构域和单纯疱疹病毒1的尾部截短的Vmw65组成，缺少转录激活结构域。在瞬时表达试验中，嵌合基因产物抑制了PRV IE启动子的转录。用该嵌合基因稳定转化的HeLa细胞系对PRV感染表现出显著抗性。在用PRV感染的转化细胞中，PRV IE基因的转录受到抑制，这表明细胞对PRV感染的抗性是由于融合蛋白干扰了IE基因的转录。
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