Peyrat M B, Soumet C, Maris P, Sanders P
Agence française de sécurité sanitaire des aliments (AFSSA), Laboratoire d'études et de recherches sur les médicaments vétérinaires et les désinfectants. BP 90203 La Haute Marche, 35302 Fougères Cedex, France.
Vet Microbiol. 2008 Apr 30;128(3-4):313-26. doi: 10.1016/j.vetmic.2007.10.021. Epub 2008 Feb 20.
Campylobacter is responsible for human bacterial enteritis and poultry meat is recognised as a primary source of infection. In slaughterhouses, cleaning and disinfection procedures are performed daily, and it has been suggested that disinfectant molecules might select for antibiotic resistant strains if shared targets or combined resistance mechanisms were involved. The aim of the study was to investigate if cleaning and disinfection procedures in poultry slaughterhouses select for antibiotic resistance in Campylobacter jejuni and C. coli and to determine the genotypes of isolates collected after cleaning and disinfection. Nine sampling visits were made to four French slaughterhouses. Samples were collected from transport crates and equipment surfaces, before and after cleaning and disinfection. Minimal inhibitory concentrations of the recovered C. jejuni and C. coli isolates to six antibiotics and two disinfectants were measured. The C. jejuni isolates collected from equipment surfaces after cleaning and disinfection were subjected to PCR-RFLP typing. Twenty-five C. jejuni isolates and 1 C. coli were recovered from equipment surfaces after cleaning and disinfection during five visits to three different slaughterhouses. Those isolates did not show an increased resistance to the tested antibiotics compared to isolates collected before cleaning and disinfection. Only one or two genotypes were recovered after cleaning and disinfection during single visits to each slaughterhouse. This observation suggests that such genotypes may be particularly adapted to survive cleaning and disinfection stress. Understanding the survival mechanisms of Campylobacter should facilitate the implementation of better-targeted strategies and reduce the public health burden associated with Campylobacter infection.
弯曲杆菌是人类细菌性肠炎的病原体，禽肉被认为是主要感染源。在屠宰场，每天都会进行清洁和消毒程序，有人提出，如果涉及共同靶点或联合耐药机制，消毒剂分子可能会选择出抗生素耐药菌株。本研究的目的是调查家禽屠宰场的清洁和消毒程序是否会选择空肠弯曲菌和大肠弯曲菌中的抗生素耐药性，并确定清洁和消毒后收集的分离株的基因型。对四家法国屠宰场进行了九次采样访问。在清洁和消毒前后，从运输箱和设备表面采集样本。测定了回收的空肠弯曲菌和大肠弯曲菌分离株对六种抗生素和两种消毒剂的最低抑菌浓度。对清洁和消毒后从设备表面收集的空肠弯曲菌分离株进行PCR-RFLP分型。在对三个不同屠宰场的五次访问中，清洁和消毒后从设备表面回收了25株空肠弯曲菌分离株和1株大肠弯曲菌分离株。与清洁和消毒前收集的分离株相比，这些分离株对测试抗生素的耐药性没有增加。在每次对每个屠宰场的单次访问中，清洁和消毒后仅回收了一两种基因型。这一观察结果表明，这些基因型可能特别适应在清洁和消毒压力下存活。了解弯曲杆菌的生存机制应有助于实施更有针对性的策略，并减轻与弯曲杆菌感染相关的公共卫生负担。
