Bergknut Magnus, Sehlin Emma, Lundstedt Staffan, Andersson Patrik L, Haglund Peter, Tysklind Mats
Department of Chemistry, Umeå University, SE-90187 Umeå, Sweden.
Environ Pollut. 2007 Jan;145(1):154-60. doi: 10.1016/j.envpol.2006.03.052. Epub 2006 May 19.
The aim of this study was to evaluate different techniques for assessing the availability of polycyclic aromatic hydrocarbons (PAHs) in soil. This was done by comparing the amounts (total and relative) taken up by the earthworm Eisenia fetida with the amounts extracted by solid-phase microextraction (SPME), semi-permeable membrane devices (SPMDs), leaching with various solvent mixtures, leaching using additives, and sequential leaching. Bioconcentration factors of PAHs in the earthworms based on equilibrium partitioning theory resulted in poor correlations to observed values. This was most notable for PAHs with high concentrations in the studied soil. Evaluation by principal component analysis (PCA) showed distinct differences between the evaluated techniques and, generally, there were larger proportions of carcinogenic PAHs (4-6 fused rings) in the earthworms. These results suggest that it may be difficult to develop a chemical method that is capable of mimicking biological uptake, and thus estimating the bioavailability of PAHs.
本研究的目的是评估评估土壤中多环芳烃（PAHs）有效性的不同技术。这是通过比较蚯蚓赤子爱胜蚓摄取的量（总量和相对量）与固相微萃取（SPME）、半透膜装置（SPMDs）、用各种溶剂混合物浸提、使用添加剂浸提以及顺序浸提所提取的量来实现的。基于平衡分配理论的蚯蚓体内PAHs生物富集因子与观测值的相关性较差。这在研究土壤中高浓度的PAHs中最为明显。主成分分析（PCA）评估显示，所评估的技术之间存在明显差异，并且一般来说，蚯蚓体内致癌PAHs（4 - 6个稠环）的比例更大。这些结果表明，可能难以开发一种能够模拟生物摄取从而估算PAHs生物有效性的化学方法。
