Burgess D E, Knoblock K F
Veterinary Research Laboratory, Montana State University, Bozeman 59717.
J Parasitol. 1989 Dec;75(6):977-80.
Sections of bovine placenta from cases of bovine trichomoniasis were examined for the presence of Tritrichomonas foetus by standard histological methods using phase-contrast microscopy and by indirect immunofluorescence assay (IFA) employing monoclonal antibodies (mAbs) specific for T. foetus. Parasites were identified readily in deparaffinized tissue up to 4 yr old by IFA with 2 mAbs previously shown to bind to the surface of living T. foetus. These results indicated that the IFA provided a rapid and specific method of identifying T. foetus in tissue sections as compared to standard histological methods.
通过使用相差显微镜的标准组织学方法以及采用针对胎儿三毛滴虫（T. foetus）的单克隆抗体（mAbs）的间接免疫荧光测定法（IFA），对牛毛滴虫病病例的牛胎盘切片进行检查，以确定是否存在胎儿三毛滴虫。通过IFA，使用之前已证明能与活的胎儿三毛滴虫表面结合的两种mAbs，在长达4年的脱蜡组织中很容易鉴定出寄生虫。这些结果表明，与标准组织学方法相比，IFA提供了一种在组织切片中鉴定胎儿三毛滴虫的快速且特异的方法。
