Schade Ulrika M, Nehmann Nina, Horny Hans-P, Prehm Peter, Delpech Bertrand, Krüger William H, Zander Axel R, Schumacher Udo
Institute for Anatomy II: Experimental Morphology, University Hospital Hamburg-Eppendorf, Martinistr. 52, D-20246 Hamburg, Germany.
Acta Histochem. 2006;108(2):141-7. doi: 10.1016/j.acthis.2006.03.018. Epub 2006 May 19.
Cell-cell and cell-matrix interactions, which are mediated by cell adhesion molecules, play a fundamental role during many cellular processes including growth, differentiation, cell migration and cancer metastasis. One molecule playing a major role in these processes is the CD44 surface receptor, which is expressed in a wide range of cells including many cells of the hemopoietic system, where it mediates the interaction with its major ligand, hyaluronate. However, little is known about CD44 and hyaluronate in bone marrow and this was investigated immunohistochemically in trephine biopsies and in cultivated human bone marrow stromal cells. In biopsy specimens, patches of hyaluronate deposition were detected in the extracellular matrix (ECM). However, most of the areas of the ECM were devoid of hyaluronate. Single mast cells and lymphocytes scattered throughout the marrow were CD44 immunopositive. Marrow-derived stromal cells (MDSC) expanded in cell culture were immunopositive for CD44, hyaluronate synthase, and hyaluronate. Hence, a marked difference between CD44 immunolocalisation and hyaluronate deposition can be observed between in situ and under cell culture conditions. Since in normal marrow in situ the number of CD44 immunopositive cells was low, interactions of CD44 and hyaluronate would appear to not to play a major role in cell adhesion in the normal bone marrow.
由细胞黏附分子介导的细胞间和细胞与基质间的相互作用，在包括生长、分化、细胞迁移和癌症转移在内的许多细胞过程中发挥着重要作用。在这些过程中起主要作用的一种分子是CD44表面受体，它在多种细胞中表达，包括造血系统的许多细胞，在这些细胞中它介导与主要配体透明质酸的相互作用。然而，关于骨髓中的CD44和透明质酸知之甚少，因此我们采用免疫组织化学方法在骨髓活检组织和培养的人骨髓基质细胞中对此进行了研究。在活检标本中，在细胞外基质（ECM）中检测到透明质酸沉积斑块。然而，ECM的大部分区域都没有透明质酸。散布在整个骨髓中的单个肥大细胞和淋巴细胞CD44免疫呈阳性。在细胞培养中扩增的骨髓来源的基质细胞（MDSC）对CD44、透明质酸合酶和透明质酸免疫呈阳性。因此，在原位和细胞培养条件下，可以观察到CD44免疫定位和透明质酸沉积之间存在明显差异。由于在正常原位骨髓中CD44免疫阳性细胞数量较少，CD44与透明质酸的相互作用似乎在正常骨髓的细胞黏附中不起主要作用。
