Ren Chengzhen, Ren Cheng-Hui, Li Likun, Goltsov Alexei A, Thompson Timothy C
Scott Department of Urology, Baylor College of Medicine, Houston, TX 77030, USA.
Genomics. 2006 Aug;88(2):163-72. doi: 10.1016/j.ygeno.2006.03.021. Epub 2006 May 22.
Our previous finding of RTVP1 (GLIPR1) as a p53 target gene with tumor suppressor functions prompted us to initiate a genome-wide sequence homology search for RTVP1/GLIPR1-like (GLIPR1L) genes. In this study we report the identification and characterization of a novel p53 target gene cluster that includes human RTVP1 (hRTVP-1) together with two GLIPR1L genes (GLIPR1L1 and GLIPR1L2) on human chromosome 12q21 and mouse Rtvp1 (mRTVP-1 or Glipr1) together with three Glipr1-like (Glipr1l) genes on mouse chromosome 10D1. GLIPR1L1 has two and GLIPR1L2 has five differentially spliced isoforms. Protein homology search revealed that hRTVP-1 gene cluster members share a high degree of identity and homology. GLIPR1L1 is testis-specific, whereas GLIPR1L2 is expressed in different types of tissues, including prostate and bladder. Like hRTVP-1, GLIPR1L1 and GLIPR1L2 are p53 target genes. The similarities of these novel p53 target gene cluster members in protein structure and their association with p53 suggest that these genes may have similar biological functions.
我们之前发现RTVP1（GLIPR1）作为具有肿瘤抑制功能的p53靶基因，这促使我们启动了一项全基因组序列同源性搜索，以寻找RTVP1/GLIPR1样（GLIPR1L）基因。在本研究中，我们报告了一个新的p53靶基因簇的鉴定和特征，该基因簇包括人类12号染色体q21上的人类RTVP1（hRTVP-1）以及两个GLIPR1L基因（GLIPR1L1和GLIPR1L2），以及小鼠10D1染色体上的小鼠Rtvp1（mRTVP-1或Glipr1）以及三个Glipr1样（Glipr1l）基因。GLIPR1L1有两种，GLIPR1L2有五种差异剪接异构体。蛋白质同源性搜索显示，hRTVP-1基因簇成员具有高度的同一性和同源性。GLIPR1L1是睾丸特异性的，而GLIPR1L2在包括前列腺和膀胱在内的不同类型组织中表达。与hRTVP-1一样，GLIPR1L1和GLIPR1L2也是p53靶基因。这些新的p53靶基因簇成员在蛋白质结构上的相似性及其与p53的关联表明，这些基因可能具有相似的生物学功能。
