Wilcock D, Lane D P
Imperial Cancer Research Fund, Clare Hall Laboratories, Hertfordshire, UK.
Nature. 1991 Jan 31;349(6308):429-31. doi: 10.1038/349429a0.
Replication of DNA occurs at discrete sites in eukaryotic cell nuclei, where replication proteins are clustered into large complexes, or 'replicases'. Similarly, viral DNA replication is a highly structured process, notably in herpes simplex virus type-1 (HSV-1; reviewed in ref. 4) in which large globular 'replication compartments' containing the viral replication machinery exist. Replicating cellular DNA redistributes to these compartments upon HSV-1 infection. We have now used antibodies raised against several cellular proteins to detect changes in their subnuclear localization on HSV-1 infection. We found that various proteins involved in cellular DNA replication move to sites of viral DNA synthesis, whereas a selection of non-replication proteins do not. The retinoblastoma protein and p53 (the products of two putative anti-oncogenes) relocate to the same sites as known DNA replication proteins, suggesting that they may be associated with DNA replication complexes in normal, uninfected cells.
DNA复制发生在真核细胞核中的离散位点，在这些位点上，复制蛋白聚集形成大的复合物，即“复制酶”。同样，病毒DNA复制也是一个高度结构化的过程，特别是在单纯疱疹病毒1型（HSV-1；参考文献4中有综述）中，存在含有病毒复制机制的大的球状“复制区室”。HSV-1感染后，正在复制的细胞DNA会重新分布到这些区室。我们现在使用针对几种细胞蛋白产生的抗体来检测HSV-1感染后它们在细胞核内定位的变化。我们发现，参与细胞DNA复制的各种蛋白会移动到病毒DNA合成位点，而一些非复制蛋白则不会。视网膜母细胞瘤蛋白和p53（两个假定的抗癌基因的产物）会重新定位到与已知DNA复制蛋白相同的位点，这表明它们可能在未感染的正常细胞中与DNA复制复合物相关联。
