Yakasai I A
Department of Pharmaceutical and Medical Chemistry Ahmadu Bello University, Zaria, Nigeria.
Eur J Drug Metab Pharmacokinet. 2006 Jan-Mar;31(1):1-4. doi: 10.1007/BF03190634.
Samples were extracted with n-hexane and the organic layer was rejected. 10 microl aliquots of the aqueous layer were injected onto the column. Amodiaquine was used as the internal standard. The UV detector response was linear over the range 0-1000 ng/ml with a correlation coefficient of 0.9987; the detection limits with respect to chloroquine and desethylchloroquine were found to be 20 ng/ml and 10 ng/ml respectively. Within-day and between-day assay variation was generally < 5%. No interference from endogenous constituents was observed. The utility of the method was demonstrated by determining chloroquine and its major metabolite, desethylchloroquine in plasma samples from six healthy human volunteers following a single oral dose of 600 mg of chloroquine. The procedure is simple and requires small volumes of plasma.
样品用正己烷萃取，弃去有机层。取10微升水层等分试样注入柱中。使用阿莫地喹作为内标。紫外检测器响应在0 - 1000 ng/ml范围内呈线性，相关系数为0.9987；氯喹和去乙基氯喹的检测限分别为20 ng/ml和10 ng/ml。日内和日间测定变异一般<5%。未观察到内源性成分的干扰。通过测定6名健康人类志愿者单次口服600 mg氯喹后血浆样品中的氯喹及其主要代谢物去乙基氯喹，证明了该方法的实用性。该方法简单，所需血浆量少。
