Chaulet J F, Robet Y, Prevosto J M, Soares O, Brazier J L
Laboratoire de Biochimie du Professeur Lemontey, Hôpital d'Instruction des Armées Desgenettes, Lyon, France.
J Chromatogr. 1993 Apr 2;613(2):303-10. doi: 10.1016/0378-4347(93)80146-u.
A high-performance liquid chromatographic method with fluorescence detection is described for the simultaneous measurement of quinine, chloroquine and mono- and bidesethylchloroquine in human plasma, erythrocytes and urine. After a liquid-solid extraction on a Bond Elut C8 cartridge, the compounds are separated on an Inertsil silica column by gradient elution; the mobile phase is a mixture of acetonitrile and methanol-25% ammonia solution (92.7:7.5, v/v). The eluent was monitored with a fluorescence detector (excitation wavelength 325 nm and emission wavelength 375 nm). The limit of detection was ca. 5 ng/ml for chloroquine and ca. 23 ng/ml for quinine. No chromatographic interferences could be detected from endogenous compounds or other antimalarial drugs. The method is accurate with inter- and intra-assay coefficients of variation lower than 7%. Hydroxychloroquine is used as an internal standard because of its structural similarity to chloroquine. The procedure requires 30 min and can be used for therapeutic drug monitoring.
本文描述了一种采用荧光检测的高效液相色谱法，用于同时测定人血浆、红细胞和尿液中的奎宁、氯喹以及单去乙基氯喹和双去乙基氯喹。在Bond Elut C8柱上进行液固萃取后，化合物在Inertsil硅胶柱上通过梯度洗脱进行分离；流动相为乙腈与甲醇 - 25%氨溶液的混合物（92.7:7.5，v/v）。用荧光检测器（激发波长325 nm，发射波长375 nm）监测洗脱液。氯喹的检测限约为5 ng/ml，奎宁的检测限约为23 ng/ml。未检测到内源性化合物或其他抗疟药物的色谱干扰。该方法准确，批间和批内变异系数低于7%。由于羟基氯喹与氯喹结构相似，故用作内标。该方法耗时30分钟，可用于治疗药物监测。
