Miyake Tsuyoshi, Uchitomi Kumiko, Zhang Ming-Yong, Kono Isato, Nozaki Nobuyuki, Sammoto Hiroyuki, Inagaki Kenji
Industrial Technology Center of Okayama Prefecture, Japan.
Biosci Biotechnol Biochem. 2006 May;70(5):1154-9. doi: 10.1271/bbb.70.1154.
Lovastatin production is dependent on the substrates provided. We investigated how several carbon and nitrogen sources in the medium affect lovastatin production by Monascus pilosus. M. pilosus required a suitable concentration of organic nitrogen peptone for high lovastatin production. As sole carbon source with peptone, although glucose strongly repressed lovastatin production, maltose was responsible for high production. Interestingly, glycerol combined with maltose enhanced lovastatin production, up to 444 mg/l in the most effective case. Moreover, an isolated mutant, in which glucose repression might be relieved, easily produced the highest level of lovastatin, 725 mg/l on glucose-glycerol-peptone medium. These observations indicate that lovastatin production by M. pilosus is regulated by strict glucose repression and that an appropriate release from this repression by optimizing medium composition and/or by a mutation(s) is required for high lovastatin production.
洛伐他汀的产量取决于所提供的底物。我们研究了培养基中的几种碳源和氮源如何影响毛红曲霉产生洛伐他汀。毛红曲霉需要适宜浓度的有机氮蛋白胨以实现高产量的洛伐他汀生产。作为与蛋白胨搭配的唯一碳源，尽管葡萄糖强烈抑制洛伐他汀的产生，但麦芽糖是实现高产量的关键。有趣的是，甘油与麦芽糖结合可提高洛伐他汀的产量，在最有效的情况下可达444毫克/升。此外，一个分离出的突变体，其可能解除了葡萄糖抑制作用，在葡萄糖 - 甘油 - 蛋白胨培养基上能轻松产生最高水平的洛伐他汀，达725毫克/升。这些观察结果表明，毛红曲霉产生洛伐他汀受到严格的葡萄糖抑制调控，并且通过优化培养基组成和/或通过突变适当解除这种抑制对于高产量的洛伐他汀生产是必需的。
