Tsukahara Masatoshi, Shinzato Naoya, Tamaki Yasutomo, Namihira Tomoyuki, Matsui Toru
Tropical Technology Center Co., 5-1 Suzaki Uruma-shi, Okinawa 901-2234, Japan.
Appl Biochem Biotechnol. 2009 Aug;158(2):476-82. doi: 10.1007/s12010-009-8553-8. Epub 2009 Feb 12.
Monascus pilosus NBRC4520 was selected for functional fermented food inoculation for its high lovastatin and low citrinin production with a deep-red color. For koji (mold rice) with high lovastatin production, separation of the growth phase and lovastatin production phase by shifting the temperature from 30 to 23 degrees C increased lovastatin production by nearly 20 times compared to temperature-constant cultivation. In addition, citrinin was not produced even in the lovastatin production phase, although the pigment was increased. With temperature-shift cultivation, 225 microg lovastatin/g dry koji was produced in 14 days without citrinin.
毛红曲霉NBRC4520因其洛伐他汀产量高、桔霉素产量低且颜色深红而被选用于功能性发酵食品接种。对于洛伐他汀产量高的曲（发霉大米），通过将温度从30℃降至23℃来分离生长阶段和洛伐他汀生产阶段，与恒温培养相比，洛伐他汀产量提高了近20倍。此外，尽管色素增加，但在洛伐他汀生产阶段甚至不产生桔霉素。通过变温培养，在14天内可生产出225微克洛伐他汀/克干曲，且无桔霉素产生。
