利用表达来自杨树的O-甲基转移酶的大肠杆菌生产三种O-甲基化七叶亭。
Production of three O-methhylated esculetins with Escherichia coli expressing O-methyltransferase from poplar.
作者信息
Kim Bong Gyu, Lee Youngshim, Hur Hor-Gil, Lim Yoongho, Ahn Joong-Hoon
机构信息
Bio/Molecular Informatics Center, Department of Bioscience and Biotechnology, Konkuk University, Seoul, Korea.
出版信息
Biosci Biotechnol Biochem. 2006 May;70(5):1269-72. doi: 10.1271/bbb.70.1269.
O-Methyltransferase, POMT-9 was expressed in Escherichia coli. HPLC analysis of reaction products revealed three peaks corresponding to isoscopoletin, scopoletin, and scoparone, and their structures were determined using NMR. Biotransformation of esculetin with E. coli expressing POMT-9 generated scopoletin, isoscopoletin, and scoparone at 30.3, 21, and 31 microM respectively. POMT-9 is the first O-methyltransferase that produces three different O-methylated products.
O-甲基转移酶POMT-9在大肠杆菌中表达。对反应产物进行高效液相色谱分析，发现了对应于异东莨菪素、东莨菪素和滨蒿内酯的三个峰，并通过核磁共振确定了它们的结构。用表达POMT-9的大肠杆菌对七叶亭进行生物转化，分别产生了30.3、21和31微摩尔的东莨菪素、异东莨菪素和滨蒿内酯。POMT-9是第一种能产生三种不同O-甲基化产物的O-甲基转移酶。
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