Murthy Sabita K, Demetrick Douglas J
Medical Genetics, Al Wasl Hospital, Dubai, United Arab Emirates.
Methods Mol Biol. 2006;319:237-59. doi: 10.1007/978-1-59259-993-6_12.
Fluorescence in situ hybridization (FISH) is a nonisotopic labeling and detection method that provides a direct way to determine the relative location or copy number of specific DNA sequences in nuclei or chromosomes. With recent advancements, this technique has found increased application in a number of research areas, including cytogenetics, prenatal diagnosis, cancer research and diagnosis, nuclear organization, gene loss and/or amplification, and gene mapping. The availability of different types of probe and the increasing number of FISH techniques has made it a widespread and diversely applied technology. Multicolor karyotyping by multicolor FISH and spectral karyotyping interphase FISH and comparative genomic hybridization allow genetic analysis of previously intractable targets. We present a brief overview of FISH technology and describe in detail methods of probe labeling and detection for different types of tissue sample, including microdissected nuclei from formalin-fixed paraffin-embedded tissue sections.
荧光原位杂交（FISH）是一种非同位素标记和检测方法，它提供了一种直接的方式来确定细胞核或染色体中特定DNA序列的相对位置或拷贝数。随着近期技术的进步，该技术在许多研究领域得到了越来越广泛的应用，包括细胞遗传学、产前诊断、癌症研究与诊断、细胞核组织、基因缺失和/或扩增以及基因图谱绘制。不同类型探针的可用性以及荧光原位杂交技术数量的不断增加，使其成为一种广泛应用且用途多样的技术。通过多色荧光原位杂交进行的多色核型分析以及光谱核型分析、间期荧光原位杂交和比较基因组杂交，使得对以前难以处理的靶点进行遗传分析成为可能。我们简要概述了荧光原位杂交技术，并详细描述了针对不同类型组织样本的探针标记和检测方法，包括从福尔马林固定石蜡包埋组织切片中显微切割得到的细胞核。
