Wagstaff Kylie M, Jans David A
Nuclear Signalling Laboratory, Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia.
Curr Med Chem. 2006;13(12):1371-87. doi: 10.2174/092986706776872871.
Cell penetrating proteins or peptides (CPPs) have the ability to cross the plasma membranes of mammalian cells in an apparently energy- and receptor-independent fashion. Although there is much debate over the mechanism by which this "protein transduction" occurs, the ability of CPPs to translocate rapidly into cells is being exploited to deliver a broad range of therapeutics including proteins, DNA, antibodies, oligonucleotides, imaging agents and liposomes in a variety of situations and biological systems. The current review looks at the delivery of many such molecules by various CPPs, and their potential therapeutic application in a wide range of areas. CPP ability to deliver different cargoes in a relatively efficient and non-invasive manner has implications as far reaching as drug delivery, gene transfer, DNA vaccination and beyond. Although many questions remain to be answered and limitations on the use of CPPs exist, it is clear that this emerging technology has much to offer in a clinical setting.
细胞穿透蛋白或肽（CPPs）能够以一种明显不依赖能量和受体的方式穿过哺乳动物细胞的质膜。尽管对于这种“蛋白质转导”发生的机制存在诸多争议，但CPPs快速转运到细胞内的能力正被用于在各种情况和生物系统中递送多种治疗剂，包括蛋白质、DNA、抗体、寡核苷酸、成像剂和脂质体。本综述探讨了各种CPPs对许多此类分子的递送情况，以及它们在广泛领域中的潜在治疗应用。CPPs以相对高效且非侵入性的方式递送不同货物的能力，其影响范围涵盖药物递送、基因转移、DNA疫苗接种等诸多方面。尽管仍有许多问题有待解答，且CPPs的使用存在局限性，但很明显，这项新兴技术在临床环境中具有很大的应用前景。
