Gupta Bhawna, Torchilin Vladimir P
Department of Pharmaceutical Sciences, Northeastern University, Boston, MA 02115, USA.
Expert Opin Drug Deliv. 2006 Mar;3(2):177-90. doi: 10.1517/17425247.3.2.177.
Cell-penetrating peptides (CPPs) are peptide vectors that can traverse through the plasma membrane barrier without breaching the integrity of the cell, and deliver various cargoes inside cell. The range of cargoes that can be delivered intracellularly by CPPs encompasses a broad variety of hydrophilic molecules, such as peptides, proteins, antibodies, imaging agents, DNA and even nanosized entities, including polymer-based systems, solid nanoparticles and liposomes. Multiple studies have focused on CPPs such as transactivating transcriptional activator peptide (TATp), penetratin, VP22, transportan and synthetic oligoarginines because of their high inherent potential as intracellular delivery vectors. However, the TATp remains the most popular CPP used for a variety of purposes. This review article attempts to bring together the available data on TAT-mediated intracellular uptake of a broad range of molecules and nanoparticles. It also considers potential practical applications of this approach.
细胞穿透肽（CPPs）是一类肽载体，能够穿越质膜屏障而不破坏细胞的完整性，并将各种货物递送至细胞内。CPPs可在细胞内递送的货物范围包括多种亲水性分子，如肽、蛋白质、抗体、成像剂、DNA，甚至纳米级实体，包括基于聚合物的系统、固体纳米颗粒和脂质体。由于其作为细胞内递送载体具有很高的内在潜力，多项研究聚焦于反式激活转录激活因子肽（TATp）、穿膜肽、VP22、转运蛋白和合成寡聚精氨酸等CPPs。然而，TATp仍然是用于多种目的的最常用的CPP。这篇综述文章试图汇集关于TAT介导的多种分子和纳米颗粒细胞内摄取的现有数据。它还考虑了这种方法的潜在实际应用。
