Nagai Takashi, Funahashi Hiroaki, Yoshioka Koji, Kikuchi Kazuhiro
National Institute of Livestock and Grassland Science, 2 Ikenodai, Tsukuba, Ibaraki, 305-0901, Japan.
Front Biosci. 2006 Sep 1;11:2565-73. doi: 10.2741/1991.
There have been intensive attempts to establish reliable in vitro maturation (IVM) and fertilization (IVF) methods in pigs. Although a great deal of progress has been made, current IVM-IVF systems still suffer from a low rate and poor quality of in vitro produced embryos . In this review, we will review the recent studies about IVM-IVF of porcine oocytes and the in vitro culture (IVC) system, especially modified in vitro production (IVP) system that produces high quality of porcine blastocysts. We then try to suggest practical ways to solve the problems mentioned above in the pigs.
人们一直在积极尝试建立可靠的猪体外成熟（IVM）和体外受精（IVF）方法。尽管已经取得了很大进展，但目前的IVM-IVF系统仍然存在体外产生胚胎的比率低和质量差的问题。在这篇综述中，我们将回顾关于猪卵母细胞IVM-IVF和体外培养（IVC）系统的最新研究，特别是能够产生高质量猪囊胚的改良体外生产（IVP）系统。然后，我们试图提出切实可行的方法来解决猪身上上述问题。
