Pollack M S, Auerbach A D, Broxmeyer H E, Zaafran A, Griffith R L, Erlich H A
Methodist Hospital, Houston, TX 77030.
Hum Immunol. 1991 Jan;30(1):45-9. doi: 10.1016/0198-8859(91)90070-p.
It has recently been demonstrated that umbilical cord blood from genotypically human leukocyte antigen (HLA)-matched donors can provide sufficient numbers of progenitor cells for hematopoietic reconstitution. This technique has been successfully applied in the treatment of two children affected with Fanconi anemia (FA). Fetal cells from the potential sibling donors were first tested to determine that the fetus was not affected with FA. Unaffected fetal cells were then tested for HLA type. Cord blood from compatible donors can be harvested at birth and used immediately or frozen for subsequent use in hematopoietic reconstitution. We now show that fetal cell DNA amplification and hybridization for DQ typing can be an important adjunct procedure to verify serologically determined HLA class II types and/or to establish class II haplotype identity with the affected sibling.
最近已证明，来自基因型人类白细胞抗原（HLA）匹配供体的脐带血可为造血重建提供足够数量的祖细胞。该技术已成功应用于两名患有范可尼贫血（FA）的儿童的治疗。首先对潜在同胞供体的胎儿细胞进行检测，以确定胎儿未患FA。然后对未受影响的胎儿细胞进行HLA分型检测。来自相容供体的脐带血可在出生时采集，立即使用或冷冻以备后续用于造血重建。我们现在表明，用于DQ分型的胎儿细胞DNA扩增和杂交可以是一种重要的辅助程序，用于验证血清学确定的HLA II类类型和/或与患病同胞建立II类单倍型一致性。
