Genovese Tiziana, Mazzon Emanuela, Menegazzi Marta, Di Paola Rosanna, Muià Carmelo, Crisafulli Concetta, Bramanti Placido, Suzuki Hisanori, Cuzzocrea Salvatore
Dipartimento Clinico Sperimentale di Medicina e Farmacologia and, Department of Neuroscience and Vision, University of Verona, Verona, Italy.
Shock. 2006 Jun;25(6):608-17. doi: 10.1097/01.shk.0000209560.54328.69.
Oxidative stress results from an oxidant/antioxidant imbalance, an excess of oxidants, and/or a depletion of antioxidants. A considerable body of recent evidence suggests that oxidative stress and exaggerated production of reactive oxygen species play a major role in several aspects of inflammation. Hypericum perforatum is a medicinal plant species containing many polyphenolic compounds, namely, flavonoids and phenolic acids. Because polyphenolic compounds have high antioxidant potential, in this study, we evaluated the effect of H. perforatum (given at 30 mg . kg (-1)) in an experimental animal model of spinal cord injury, which was induced by the application of vascular clips to the dura via a four-level T5 through T8 laminectomy. The degree of (a) spinal cord inflammation and tissue injury (histological score), (b) nitrotyrosine, (c) poly(adenosine diphosphate-ribose), (d) neutrophils infiltration, and (e) the activation of signal transducer and activator transcription 3 was markedly reduced in spinal cord tissue obtained from H. perforatum extract-treated mice. We have also demonstrated that H. perforatum extract significantly ameliorated the recovery of limb function.
氧化应激源于氧化剂/抗氧化剂失衡、氧化剂过量和/或抗氧化剂耗竭。最近大量证据表明，氧化应激和活性氧的过度产生在炎症的多个方面起主要作用。贯叶连翘是一种药用植物，含有许多多酚类化合物，即黄酮类化合物和酚酸。由于多酚类化合物具有较高的抗氧化潜力，在本研究中，我们评估了贯叶连翘（以30 mg·kg⁻¹给药）在脊髓损伤实验动物模型中的作用，该模型是通过四级T5至T8椎板切除术对硬脑膜应用血管夹诱导的。在从贯叶连翘提取物处理的小鼠获得的脊髓组织中，（a）脊髓炎症和组织损伤程度（组织学评分）、（b）硝基酪氨酸、（c）聚（二磷酸腺苷-核糖）、（d）中性粒细胞浸润以及（e）信号转导和转录激活因子3的激活均显著降低。我们还证明，贯叶连翘提取物显著改善了肢体功能的恢复。
