Paterniti Irene, Genovese Tiziana, Crisafulli Concetta, Mazzon Emanuela, Di Paola Rosanna, Galuppo Maria, Bramanti Placido, Cuzzocrea Salvatore
Department of Clinical and Experimental Medicine and Pharmacology, School of Medicine, University of Messina, Torre Biologica, Gazzi, Messina, Italy.
Naunyn Schmiedebergs Arch Pharmacol. 2009 Aug;380(2):179-92. doi: 10.1007/s00210-009-0414-z. Epub 2009 Apr 1.
In this study, we evaluated the effect of green tea extract (that was administered 25 mg/kg intraperitoneal at 1 and 6 h after injury) in experimental animal model of spinal cord injury. The spinal cord trauma was induced by the application of vascular clips to the dura via a four-level T5-T8 laminectomy. Spinal cord injury in mice resulted in severe trauma characterised by oedema, neutrophilic infiltration and apoptosis. Also, immunohistochemical examination demonstrated a marked increase in immune reactivity for nitrotyrosine. All parameters of inflammation were attenuated by green tea extract. The degree of spinal cord inflammation, nitrotyrosine, poli (ADP-ribosio) synthetase (PARS) and neutrophilic infiltration was markedly reduced. Green tea extract significantly ameliorated the recovery of limb function. Values shown are mean +/- SE mean of ten mice for each group. *p < 0.01 versus sham, degrees p < 0.01 versus spinal cord injury. Taken together, our results clearly demonstrate that green tea extract treatment ameliorates spinal cord injury oxidative stress.
在本研究中，我们评估了绿茶提取物（在损伤后1小时和6小时以25毫克/千克的剂量腹腔注射）对脊髓损伤实验动物模型的影响。通过四级T5 - T8椎板切除术对硬脑膜应用血管夹来诱导脊髓创伤。小鼠脊髓损伤导致严重创伤，其特征为水肿、中性粒细胞浸润和细胞凋亡。此外，免疫组化检查显示硝基酪氨酸的免疫反应性显著增加。绿茶提取物减轻了所有炎症参数。脊髓炎症程度、硝基酪氨酸、聚（ADP - 核糖）合成酶（PARS）和中性粒细胞浸润均显著降低。绿茶提取物显著改善了肢体功能的恢复。所示数值为每组十只小鼠的平均值±标准误平均值。*与假手术组相比，p < 0.01；与脊髓损伤组相比，度数p < 0.01。综上所述，我们的结果清楚地表明，绿茶提取物治疗可减轻脊髓损伤的氧化应激。
