[低分化胃癌患者血清生物标志物的鉴定]
[Identification of biomarkers in the serum of the patients with poorly differentiated gastric cancer].
作者信息
Li Wei, Liu Bing-ya, Li Jian-fang, Chen Xue-hua, Hao Jian-hong, Cai Qu, Gu Qin-long, Zhu Zheng-gang, Lin Yan-zhen
机构信息
Department of Surgery, Ruijin Hospital, Shanghai Second Medical University, Shanghai Institute of Digestive Surgery, Shanghai 200025, China.
出版信息
Zhonghua Wei Chang Wai Ke Za Zhi. 2006 May;9(3):253-6.
OBJECTIVE
To investigate the application of proteomics in the field of serology,and to screen the differential expression proteins related with poorly differentiated gastric carcinoma.
METHODS
Two-dimensional electrophoresis (2-DE) was applied to segregate the total proteins in the serum form gastric cancer patients and health volunteers. After staining,the differential expression proteins were analyzed using PDQuest software,and identified using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS).
RESULTS
Electrophoresis figures with high resolution and reproducibility were obtained. Six differential expression proteins were found only in the serum from gastric cancer patients, while four other proteins from healthy volunteers.
CONCLUSIONS
Protein expression is differential in the serum from the gastric cancer patients and health volunteers. It is hopeful to find the biomarkers related with poorly differentiated gastric carcinoma using proteomics.
目的
探讨蛋白质组学在血清学领域的应用，筛选与低分化胃癌相关的差异表达蛋白。
方法
应用双向电泳（2-DE）分离胃癌患者和健康志愿者血清中的总蛋白。染色后，使用PDQuest软件分析差异表达蛋白，并采用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）进行鉴定。
结果
获得了分辨率高且可重复的电泳图谱。仅在胃癌患者血清中发现6种差异表达蛋白，而在健康志愿者血清中发现另外4种蛋白。
结论
胃癌患者和健康志愿者血清中的蛋白质表达存在差异。利用蛋白质组学有望找到与低分化胃癌相关的生物标志物。
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