Goecke I A, Bonilla S, Marusic E T, Alvo M
Department of Physiology and Biophysics, Faculty of Medicine, University of Chile, Santiago.
Kidney Int. 1991 Jan;39(1):39-43. doi: 10.1038/ki.1991.5.
Translocation of potassium to the intracellular compartment is impaired in advanced chronic renal failure. The purpose of this study was to evaluate the role of endogenous insulin in the disposal of an oral potassium load in uremia. Experiments were done on male Sprague-Dawley rats. Chronic renal failure (CRF) was induced by 3/4 nephrectomy. The results show that the addition of oral glucose to a potassium load was more effective in the translocation of potassium to the intracellular compartment in uremic animals. Further, suppression of endogenous insulin secretion with somatostatin caused a much higher increase in plasma potassium (K) of uremic rats (1.09 +/- 0.15 mEq/liter in CRF vs. 0.28 +/- 0.03 mEq/liter in control). Experiments to assess the activity of the Na pump were done in soleus muscles derived from these animals. Although a 50% reduction of the basal Na pump activity was found in the uremic muscles, the addition of insulin 100 mU/ml caused a relatively greater stimulation of ouabain-sensitive 86Rb uptake in the uremic muscle as compared to the control tissue (203% vs. 77% increment). These data suggest a greater sensitivity to insulin action on extrarenal potassium disposal in uremia.
在晚期慢性肾衰竭中，钾向细胞内区室的转运受损。本研究的目的是评估内源性胰岛素在尿毒症患者口服钾负荷处理中的作用。实验在雄性Sprague-Dawley大鼠身上进行。通过3/4肾切除术诱导慢性肾衰竭（CRF）。结果表明，在尿毒症动物中，向钾负荷中添加口服葡萄糖对钾向细胞内区室的转运更有效。此外，用生长抑素抑制内源性胰岛素分泌导致尿毒症大鼠血浆钾（K）升高幅度大得多（CRF组为1.09±0.15 mEq/升，对照组为0.28±0.03 mEq/升）。在源自这些动物的比目鱼肌中进行了评估钠泵活性的实验。虽然在尿毒症肌肉中发现基础钠泵活性降低了50%，但与对照组织相比，添加100 mU/ml胰岛素导致尿毒症肌肉中哇巴因敏感的86Rb摄取受到相对更大的刺激（增加203%对77%）。这些数据表明，尿毒症患者对胰岛素作用于肾外钾处理更为敏感。
