[四种酶联免疫吸附测定技术（两种用于IgM，两种用于IgG）在Q热暴发血清学诊断中的应用]
[Application of four ELISA techniques (two for IgM and two for IgG) for serological diagnosis of an outbreak of Q fever].
作者信息
Sanz Juan Carlos, de los Ríos Rosa, Martín Fernando, Tébar M Angeles, Jado Isabel, Anda Pedro
机构信息
Laboratorio Regional de Salud Pública, Instituto de Salud Pública, Comunidad de Madrid, Spain.
出版信息
Enferm Infecc Microbiol Clin. 2006 Mar;24(3):178-81. doi: 10.1157/13086551.
OBJECTIVE
To evaluate four ELISAs (commercialized by Panbio and Serion) for IgM and IgG detection in an outbreak of Q fever.
METHODS
Fifty-three serum samples corresponding to 29 persons were processed. An indirect immunofluorescence assay was used as the reference method.
RESULTS
The sensitivity and specificity for IgM were 100% and 74% (Panbio), and 91% and 88% (Serion). IgG sensitivity was 72% (Panbio) and 89% (Serion). The specificity of both techniques for IgG was 97%.
CONCLUSIONS
Both ELISAs seem to be good IgM screening methods for use during Q fever outbreaks.
目的
评估四种酶联免疫吸附测定法（由Panbio和Serion公司商业化生产）用于Q热暴发时IgM和IgG检测的效果。
方法
对来自29人的53份血清样本进行处理。采用间接免疫荧光测定法作为参考方法。
结果
IgM的敏感性和特异性分别为：Panbio公司产品为100%和74%，Serion公司产品为91%和88%。IgG的敏感性分别为：Panbio公司产品为72%，Serion公司产品为89%。两种技术检测IgG的特异性均为97%。
结论
在Q热暴发期间，两种酶联免疫吸附测定法似乎都是用于IgM筛查的良好方法。
Zotero 插件