Wang Yue-Hua
Department of Biological Engineering, Chengdu University, Chengdu 610160, China.
Zhongguo Zhong Yao Za Zhi. 2006 Mar;31(6):448-51.
This paper aimed at improving existing breeds of Phellodendron chinense and enriching the resources of heredity breeding.
The polyploid of P. chinense was obtained using calli, seeds and buds, which were treated with colchicines solution.
The polyploid of P. chinense was obtained using calli with stem treated with 0.2% colchicines medium, the inducing rate reached 12.45%. Compared with the morphology and chromosomal number the polyploid induction was proved to be effective.
It was an effective biotechnological way to use colchicines to induce polyploid on the different explants of P. chinense.
本文旨在改良现有黄柏品种，丰富遗传育种资源。
采用愈伤组织、种子和芽，用秋水仙素溶液处理，获得黄柏多倍体。
用0.2%秋水仙素培养基处理茎段愈伤组织获得黄柏多倍体，诱导率达12.45%。通过形态学和染色体数目比较，证明多倍体诱导有效。
利用秋水仙素对黄柏不同外植体进行多倍体诱导是一种有效的生物技术方法。
