Duan Ying-Zi, Ke Shao-Ying, Cao Jing, Niu Ying-Ze, Peng Chao-Zhong
Department of Biology, Tangshan Teacher's College, Tangshan 063000, China.
Zhongguo Zhong Yao Za Zhi. 2006 Mar;31(6):445-8.
To explore the technique of induction of polyploidy in Salvia bowleyana by colchicine treatment.
The three kinds of explant of bud, leaf and calli were induced by colchicine treatment.
The induction effects were better when the calli was treated by colchicines (15 mg x L(-1)) and the leaf was pre-cultured for one week. The doubling rate was 33.33%, while the majority were wholy doubled plants, and the leaves were thicker and broader, the color was darker, the root was thicker and the stoma size was obviously bigger than the diploid plants. The number of chromosome were 8 to 64. Isoenzyme analysis showed that the enzyme activities between the polyploid and the diploid plants were quite different.
Induction of polyploidy by colchicine treatment is efficacious. The part of the doubled plants were identified as homologmous tetraploids.
探索用秋水仙素处理诱导鲍氏鼠尾草多倍体的技术。
用秋水仙素处理芽、叶和愈伤组织这三种外植体。
用15mg·L⁻¹秋水仙素处理愈伤组织且叶片预培养一周时诱导效果较好。加倍率为33.33%，多数为完全加倍植株，其叶片更厚更宽，颜色更深，根更粗，气孔大小明显大于二倍体植株。染色体数目为8至64条。同工酶分析表明多倍体植株和二倍体植株之间的酶活性有很大差异。
秋水仙素处理诱导多倍体是有效的。部分加倍植株被鉴定为同源四倍体。
