Lee Chungnan, Wu Jain-Shing, Shiue Yow-Ling, Liang Hong-Long
Department of Computer Science and Engineering, National Sun Yat-Sen University, Kaohsiung, Taiwan.
Appl Bioinformatics. 2006;5(2):99-109. doi: 10.2165/00822942-200605020-00004.
This study presents a novel application, MultiPrimer, to assist users to design multiplex PCR primers. MultiPrimer adopts an algorithm based on a genetic algorithm and can efficiently solve the multi-objective optimisation problem. A match pattern model is proposed to speed up examination of the specificity constraint. MultiPrimer is able to find a set of primer pairs simultaneously for a set of gene family sequences and multiple targets for a single cDNA sequence. A public version of the MultiPrimer software is available from http://edith.cse.nsysu.edu.tw/new/software/MultiPrimer.htm.
本研究提出了一种新型应用程序MultiPrimer，以协助用户设计多重PCR引物。MultiPrimer采用基于遗传算法的算法，能够有效解决多目标优化问题。提出了一种匹配模式模型以加速特异性约束的检查。MultiPrimer能够同时为一组基因家族序列找到一组引物对，并为单个cDNA序列找到多个靶标。可从http://edith.cse.nsysu.edu.tw/new/software/MultiPrimer.htm获得MultiPrimer软件的公开版本。
