Fernandes Rohan, Skiena Steven
Computer Science Department, Rutgers, State University of New Jersey, Piscataway, NJ, USA.
Methods Mol Biol. 2007;402:305-14. doi: 10.1007/978-1-59745-528-2_15.
To construct full-genome spotted microarrays, a large number of PCR primers that amplify the required DNA need to be synthesized. We describe an algorithmic technique that allows one to use fewer primers to achieve this goal. This can reduce the expense of constructing full-genome spotted microarrays considerably. PCR primers are usually designed, so that each primer occurs uniquely in the genome. This condition is unnecessarily strong for selective amplification, because only the primer pair associated with each amplification needs be unique. We also describe the interface to our software, MultiPrimer, that computes a small set of primers for amplification of a given gene set.
为构建全基因组点阵微阵列，需要合成大量用于扩增所需DNA的PCR引物。我们描述了一种算法技术，可使人们用较少的引物实现这一目标。这能大幅降低构建全基因组点阵微阵列的成本。PCR引物通常设计为在基因组中唯一出现。对于选择性扩增而言，这一条件并非必要的严格，因为仅与每次扩增相关的引物对需要是唯一的。我们还描述了我们的软件MultiPrimer的界面，该软件可计算出用于扩增给定基因集的一小套引物。
