Zhan Li-ying, Xia Zhong-yuan, Chen Chang, Wang Xiao-yuan
Department of Anesthesiology, Renmin Hospital, Wuhan University, Wuhan 430060, China.
Chin J Traumatol. 2006 Jun;9(3):181-6.
To investigate the effect of Radix Paeoniae Rubra (RPR) on the expression of heme oxygenase (HO-1) and induced nitric oxide synthase (iNOS) in endotoxin-induced acute lung injury in rats and its protective mechanism.
Forty Wistar rats were divided randomly into 5 groups with 8 rats in each group: saline control group (NS group), lipopolysaccharide group (LPS group), RPR-treatment group, RPR-prevention group and Hemin group. The effect of RPR on protein content, the ratio of neutrophiles in bronchoalveolar lavage fluid, malondialdehyde (MDA) content in the lung and the activity of serum NO were observed. Arterial blood was drawn for blood-gas analysis. The expression of HO-1 and iNOS in lung tissues was detected by immunohistochemistry and morphometry computer image analysis. The histological changes of the lung were observed under light microscope.
Compared with that in NS group, the expression of HO-1 and iNOS was markedly increased in LPS group (P<0.01). In RPR-treatment, RPR-prevention, and Hemin groups, the expression of iNOS was significantly lower, while the expression of HO-1 was higher than that in LPS group (P<0.05). The protein content, the ratio of neutrophiles in bronchoalveolar lavage fluid, the content of MDA and the activity of serum NO in LPS group were significantly higher than those in NS group (P<0.01). There was a significant decrease in the level of arterial bicarbonate and partial pressure of oxygen in the LPS group (P<0.01); these parameters of lung injury however, were significantly lower in RPR-treatment, RPR-prevention, and Hemin groups than LPS group (P<0.05 or P<0.01). The pathologic changes of lung tissues were substantially attenuated in RPR-treatment, RPR-prevention, and Hemin groups than LPS group.
The high expression of HO-1 reflects an important protective function of the body during lipopolysaccharide-induced acute lung injury. The protective effect of RPR on lipopolysaccharide-induced acute lung injury is related to the inhibition of iNOS expression and the induction of HO-1 expression.
探讨赤芍对内毒素诱导的大鼠急性肺损伤中血红素加氧酶（HO-1）和诱导型一氧化氮合酶（iNOS）表达的影响及其保护机制。
40只Wistar大鼠随机分为5组，每组8只：生理盐水对照组（NS组）、脂多糖组（LPS组）、赤芍治疗组、赤芍预防组和血红素组。观察赤芍对支气管肺泡灌洗液中蛋白质含量、中性粒细胞比例、肺组织中丙二醛（MDA）含量及血清一氧化氮（NO）活性的影响。采集动脉血进行血气分析。采用免疫组织化学和形态计量学计算机图像分析检测肺组织中HO-1和iNOS的表达。在光学显微镜下观察肺组织的组织学变化。
与NS组相比，LPS组HO-1和iNOS的表达显著增加（P<0.01）。在赤芍治疗组、赤芍预防组和血红素组中，iNOS的表达显著降低，而HO-1的表达高于LPS组（P<0.05）。LPS组支气管肺泡灌洗液中蛋白质含量、中性粒细胞比例、MDA含量及血清NO活性均显著高于NS组（P<0.01）。LPS组动脉血碳酸氢盐水平和氧分压显著降低（P<0.01）；然而，赤芍治疗组、赤芍预防组和血红素组的这些肺损伤参数均显著低于LPS组（P<0.05或P<0.01）。与LPS组相比，赤芍治疗组、赤芍预防组和血红素组肺组织的病理变化明显减轻。
HO-1的高表达反映了机体在脂多糖诱导的急性肺损伤中的重要保护作用。赤芍对脂多糖诱导的急性肺损伤的保护作用与抑制iNOS表达和诱导HO-1表达有关。
