Xie Wen-guang, Ma Xiao-chang, Shao Ning-sheng
Affiliated Hospital, North Sichuan Medical College, Nanchong, Sichuan.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2005 Jun;25(6):520-4.
To study the effect of red peony root (RPR) on serum proteome in rat suffering from noxious heat with blood stasis Syndrome (NH-BS).
The differences of serum proteome among rats in four groups, treated with lipopolysaccharide (LPS), RPR, LPS + RPR and saline respectively, were analyzed by bi-dimensional electrophoresis (2DE) assay. LPS was administered by intravenous injection and RPR by oral intake.
(1) Serum of rats with LPS induced NH-BS showed significant changes in volume of serum protein (xPr) in 13 points on 2DE collagen, the volume of xPr 16 and 19 were significantly lower, volume of xPr 1, 2, 3, 4, 6, 7, 8, 9, 11, 12 and 23 were significantly higher respectively, as compared with those in the normal control group. (2) After being treated with RPR, the increased volume of xPr 1, 2, 3, 4 and 9 significantly decreased, and the decreased xPr 16 significantly increased, with xPr 2, 3 restored to normal level but the xPr16 still lower and xPr 1, 4, 9 higher than those in the normal group. RPR showed interaction with LPS on xPr 1, 3, 9, and 16. (3) For xPr 19, the interaction of RPR with LPS might be synergistic. (4) In the group treated with RPR, volumes of xPr 13 and 14 were significantly higher and those of 15, 17 were significantly lower than those in the normal group respectively, but the similar changes didn't found in the LPS group.
The molecular basis of therapeutic effect of RPR on NH-BS might be through the regulation of xPr 1, 2, 3, 4, 9 and 16.
研究赤芍对热瘀证大鼠血清蛋白质组的影响。
采用二维电泳（2DE）分析分别用脂多糖（LPS）、赤芍、LPS + 赤芍及生理盐水处理的四组大鼠血清蛋白质组的差异。LPS通过静脉注射给药，赤芍通过口服给药。
（1）LPS诱导的热瘀证大鼠血清在2DE胶上13个点的血清蛋白量（xPr）有显著变化，与正常对照组相比，xPr 16和19的量显著降低，xPr 1、2、3、4、6、7、8、9、11、12和23的量分别显著升高。（2）赤芍处理后，xPr 1、2、3、4和9升高的量显著降低，xPr 16降低的量显著升高，xPr 2、3恢复至正常水平，但xPr 16仍低于正常组，xPr 1、4、9高于正常组。赤芍与LPS在xPr 1、3、9和16上存在相互作用。（3）对于xPr 19，赤芍与LPS的相互作用可能具有协同性。（4）在赤芍处理组中，xPr 13和14的量分别显著高于正常组，15、17的量显著低于正常组，但LPS组未发现类似变化。
赤芍治疗热瘀证疗效的分子基础可能是通过调节xPr 1、2、3、4、9和16实现的。
