Rutherford R B, Foster B L, Bammler T, Beyer R P, Sato S, Somerman M J
Department of Oral Biology, School of Dentistry, University of Washington, Box 357132, Seattle, WA 98195-7132, USA.
J Dent Res. 2006 Jun;85(6):505-9. doi: 10.1177/154405910608500605.
Genetic data from humans and mice reveal that the formation of cementum is sensitive to intra- and extracellular phosphate/pyrophosphate distribution. The intracellular molecular pathways whereby altered levels of extracellular phosphate concentration may affect cementum formation have not been elucidated. To initiate inquiry, we have studied the temporal effects of extracellular phosphate on global patterns of gene expression in a line of immortalized mouse cementoblasts. Total RNA from cultured cementoblasts treated with 5 mM inorganic phosphate over a designated time period, from 1-48 hrs, was analyzed for global patterns of gene expression by means of DNA microarrays representing the complete mouse genome. Analyses of significant hybridization signals indicated that 5 mM extracellular phosphate alters the expression of genes comprising several gene ontology (GO) groups, including transcription factor activity and Wnt signaling.
来自人类和小鼠的遗传数据表明，牙骨质的形成对细胞内和细胞外磷酸盐/焦磷酸盐的分布敏感。细胞外磷酸盐浓度改变影响牙骨质形成的细胞内分子途径尚未阐明。为了展开探究，我们研究了细胞外磷酸盐对永生化小鼠成牙骨质细胞系中基因表达全局模式的时间效应。在指定时间段（1至48小时）用5 mM无机磷酸盐处理培养的成牙骨质细胞，提取总RNA，通过代表完整小鼠基因组的DNA微阵列分析基因表达的全局模式。对显著杂交信号的分析表明，5 mM细胞外磷酸盐改变了包括转录因子活性和Wnt信号在内的几个基因本体（GO）组的基因表达。
