Lee Young-Mi, Park Tae-Jin, Jung Sang-Oun, Seo Jung Soo, Park Heum Gi, Hagiwara Atsushi, Yoon Yong-Dal, Lee Jae-Seong
Department of Environmental Science, Graduate School, Hanyang University, Seoul 133-791, South Korea.
Mar Environ Res. 2006 Jul;62 Suppl:S219-23. doi: 10.1016/j.marenvres.2006.04.050. Epub 2006 Apr 18.
To investigate the impacts of marine pollution on aquatic organisms, we tested the intertidal copepod Tigriopus japonicus as a model species. To analyze the copepods' responses to endocrine-disrupting chemicals (EDCs), we exposed them to two different chemicals: 4,4'-octylphenol (4,4'-OP, 12.5-100 microg/L for 2 h) and polychlorinated biphenyl (PCB, 6.25-25 microg/L for two days). 4,4'-OP was toxic, although exposure time was limited to 2h. After extracting total RNA from the exposed T. japonicus, we performed reverse transcriptase-polymerase chain reaction (RT-PCR) to determine gene expression patterns following chemical exposure. To analyze the gene expression of T. japonicus, we used glutathione S-transferase with GAPDH as an internal control. Of the genes tested using EDC-exposed samples, 4,4'-OP induced upregulation of the glutathione S-transferase (GST) gene, while PCB caused downregulation of the GST gene. These results suggest that the two EDCs act in different manners in T. japonicus.
为了研究海洋污染对水生生物的影响，我们以潮间带桡足类日本虎斑猛水蚤作为模式物种进行了测试。为了分析桡足类对内分泌干扰化学物质（EDCs）的反应，我们将它们暴露于两种不同的化学物质中：4,4'-辛基酚（4,4'-OP，12.5 - 100微克/升，暴露2小时）和多氯联苯（PCB，6.25 - 25微克/升，暴露两天）。尽管暴露时间限制在2小时，但4,4'-OP具有毒性。从暴露后的日本虎斑猛水蚤中提取总RNA后，我们进行了逆转录聚合酶链反应（RT-PCR）以确定化学物质暴露后的基因表达模式。为了分析日本虎斑猛水蚤的基因表达，我们使用谷胱甘肽S-转移酶，并以甘油醛-3-磷酸脱氢酶（GAPDH）作为内参。在用暴露于EDCs的样本测试的基因中，4,4'-OP诱导了谷胱甘肽S-转移酶（GST）基因的上调，而PCB导致了GST基因的下调。这些结果表明，这两种EDCs在日本虎斑猛水蚤中的作用方式不同。
