Tomita Y, Akiyama M, Shimizu H
Department of Dermatology, Hokkaido University Graduate School of Medicine, N15 W7 Sapporo 060-8638, Japan.
J Dermatol Sci. 2006 Aug;43(2):105-15. doi: 10.1016/j.jdermsci.2006.03.012. Epub 2006 May 24.
It is known that platelet-derived growth factor (PDGF) receptors are expressed in hair follicle (HF) epithelium.
The aim of the present study was to clarify the effects of PDGF-AA and -BB on the cyclic growth of HFs.
PDGF-AA or -BB was injected into the dorsal skin of C3H mice during the second telogen phase once daily for five consecutive days, or PDGF-AA or -BB dissolved in hyaluronic acid was injected only once. In order to confirm the effects of different PDGF isoforms, anti-PDGF-AA antibody or anti-PDGF-BB antibody was injected just after each injection of PDGF-AA or -BB. In addition, anti-PDGF antibodies were injected into the skin of C3H mice during the second anagen phase once daily for 5 days. We studied expression of signaling molecules in the skin where anagen phase had been induced by PDGF injection by real-time RT-PCR.
Both PDGF-AA and -BB injection experiments immediately induced the anagen phase of the hair growth cycle at the injection sites. The induction of anagen was interfered by anti-PDGF antibody treatment. Real-time RT-PCR using extracted RNA from the PDGF injected sites of skin samples showed upregulated expression of HF differentiation-related key signaling molecules, Sonic hedgehog (Shh), Lef-1 and Wnt5a.
These results indicate that both PDGF-AA and -BB are involved in the induction and maintenance of the anagen phase in the mouse hair cycle. Local application of PDGF-AA and -BB might therefore prove to be an effective treatment option for alopecia associated with early catagen induction and elongated telogen phase.
已知血小板衍生生长因子（PDGF）受体在毛囊（HF）上皮中表达。
本研究旨在阐明PDGF - AA和 - BB对毛囊周期性生长的影响。
在C3H小鼠的第二个休止期，将PDGF - AA或 - BB每天注射到背部皮肤一次，连续注射五天，或者仅注射一次溶解在透明质酸中的PDGF - AA或 - BB。为了确认不同PDGF异构体的作用，在每次注射PDGF - AA或 - BB后立即注射抗PDGF - AA抗体或抗PDGF - BB抗体。此外，在第二个生长期，将抗PDGF抗体每天注射到C3H小鼠的皮肤中，持续5天。我们通过实时逆转录聚合酶链反应（RT - PCR）研究了在通过注射PDGF诱导出生长期的皮肤中信号分子的表达。
PDGF - AA和 - BB注射实验均立即在注射部位诱导了毛发生长周期的生长期。抗PDGF抗体处理干扰了生长期的诱导。使用从皮肤样本的PDGF注射部位提取的RNA进行实时RT - PCR显示，毛囊分化相关关键信号分子音猬因子（Shh）、淋巴细胞增强因子1（Lef - 1）和无翅型MMTV整合位点家族成员5A（Wnt5a）的表达上调。
这些结果表明，PDGF - AA和 - BB均参与了小鼠毛发周期中生长期的诱导和维持。因此，局部应用PDGF - AA和 - BB可能被证明是治疗与早期退行期诱导和延长休止期相关的脱发的有效选择。
