Lindner G M, Wright R W
Pacific Embryonics 1510 S. Mills Ave., #118, Lodi, CA 95240 USA.
Theriogenology. 1983 Oct;20(4):407-16. doi: 10.1016/0093-691x(83)90201-7.
The following paper briefly reviews the morphology of the bovine embryo and presents a retrospective analysis of bovine embryo transfer results accumulated from April to December of 1982 at a commercial embryo transfer center. Of particular interests were bovine embryo morphology, assessment of embryo quality, and recipient-donor, recipient-embryo synchrony requirements. Embryos were recovered from superovulated donors five to nine days after estrus (estrus = day O). All embryos were individually examined at 200X for cell stage of development and embryo quality. Embryos were nonsurgically transferred to recipients that were within two days of estrous cycle synchrony with the donor. Attempts were made to synchronize estimated developmental age of embryos to the day of the recipient cycle. A high degree of variability was observed in morphological development and embryo quality within and among donors. Embryo recovery in individual donors resulted in a wide range of embryonic cell stages, often differing in estimated developmental ages from 24 to 48 hours. A total of 783 embryos were transferred, resulting in 308 pregnancies. Stage of embryonic development (16-cell through hatched blastocyst) had little effect on pregnancy rates. Embryo quality was a more accurate predictor of success. Embryos of excellent, good, fair and poor categories resulted in 45%, 44%, 27% and 20% pregnancy rates, respectively. Recipient-donor estrous cycle synchrony of two days in either direction did not significantly alter pregnancy rates. However, 88% of 258 pregnancies (584 total transfers) occurred with a +/-1 day recipient-embryo synchrony compared to 74% based on +/-1 day recipient-donor cycle synchrony (P<0.001). Results suggest that transfer of bovine embryos based on synchrony between day of recipient cycle and state of embryonic development provides higher pregnancy rates than transfers based on recipient-donor cycle synchrony.
以下论文简要回顾了牛胚胎的形态，并对1982年4月至12月在一家商业胚胎移植中心积累的牛胚胎移植结果进行了回顾性分析。特别感兴趣的是牛胚胎形态、胚胎质量评估以及受体 - 供体、受体 - 胚胎同步要求。在发情期（发情日 = 第0天）后五至九天从超排供体中回收胚胎。所有胚胎均在200倍放大倍数下单独检查发育的细胞阶段和胚胎质量。胚胎通过非手术方式移植到与供体发情周期同步在两天内的受体中。尝试使胚胎的估计发育年龄与受体周期的日期同步。在供体内部和之间观察到形态发育和胚胎质量存在高度变异性。单个供体的胚胎回收导致胚胎细胞阶段范围广泛，估计发育年龄通常相差24至48小时。总共移植了783个胚胎，导致308例妊娠。胚胎发育阶段（16细胞至孵化囊胚）对妊娠率影响不大。胚胎质量是成功的更准确预测指标。优秀、良好、中等和差类别的胚胎妊娠率分别为45% 和44%、27% 和20%。受体 - 供体发情周期在任何一个方向上同步两天并没有显著改变妊娠率。然而，258例妊娠中的88%（共584次移植）发生在受体 - 胚胎同步为±1天的情况下，而基于受体 - 供体周期同步±1天的妊娠率为74%（P<0.001）。结果表明，基于受体周期日与胚胎发育状态同步进行牛胚胎移植比基于受体 - 供体周期同步进行移植能提供更高的妊娠率。
