Chumchai Rujira, Ratsiri Thanaporn, Ratchamak Ruthaiporn, Vongpralub Thevin, Boonkum Wuttigrai, Chankitisakul Vibuntita
Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand.
The Research and Development Network Center of Animal Breeding and Omics, Khon Kaen University, Khon Kaen, Thailand.
Anim Reprod. 2021 Nov 4;18(3):e20210053. doi: 10.1590/1984-3143-AR2021-0053. eCollection 2021.
The conventional method of ovarian superstimulation requires multiple injections of gonadotropins which is time-consuming and may be stressful for the cows. This study was designed to determine whether a single epidural injection of FSH (EI group) would induce the superovulatory response in the Thai-Holstein crossbreed and evaluate FSH plasma hormone concentrations. Eight cows (replication = 3; =24) were assigned to one of 2 treatments in switch back design. Control group (=12): cows were received 400 mg FSH twice daily by intramuscularly for 4 days (80, 80, 60, 60, 40, 40, 20 and 20 mg), EI group (=12): cows were received 400 mg FSH by single epidural injection. Data were collected in term of ovarian follicle responses, superovulatory responses, ova/embryo collection. FSH concentrations were examined using ELISA. The total follicular responses during oestrus were not different between treatments; however, the large follicles were less frequent ( < 0.01) while the medium follicle sizes were higher ( < 0.05) in the EI group. The plasma concentration of FSH in EI was dramatically increased within 2 hours before decreasing sharply thereafter ( < 0.01) and did not remain above baseline after 10 hours of administration. The embryo quality was better in the control than the EI groups ( < 0.05). Interestingly, the number of ovulation cysts in the EI group was 50%. The ovarian responses and embryo quality in the cows with cysts were worse compared with the non-cyst groups ( < 0.05). In conclusion, alternative protocols decreased the superovulatory response and increased poor embryo quality in Thai-Holstein crossbred. Also, the incidence of ovarian follicular cysts is higher in the EI group.
传统的卵巢超刺激方法需要多次注射促性腺激素，这既耗时又可能给奶牛带来压力。本研究旨在确定单次硬膜外注射促卵泡素（EI组）是否能在泰国-荷斯坦杂交奶牛中诱导超排卵反应，并评估促卵泡素的血浆激素浓度。八头奶牛（重复次数 = 3；总数 = 24）采用交叉设计被分配到两种处理之一。对照组（n = 12）：奶牛每天两次肌肉注射400毫克促卵泡素，共4天（80、80、60、60、40、40、20和20毫克），EI组（n = 12）：奶牛通过单次硬膜外注射接受400毫克促卵泡素。收集有关卵巢卵泡反应、超排卵反应、卵子/胚胎采集的数据。使用酶联免疫吸附测定法检测促卵泡素浓度。发情期的总卵泡反应在各处理之间没有差异；然而，EI组的大卵泡频率较低（P < 0.01），而中等卵泡大小的频率较高（P < 0.05）。EI组促卵泡素的血浆浓度在2小时内急剧升高，此后急剧下降（P < 0.01），给药10小时后未保持在基线以上。对照组的胚胎质量优于EI组（P < 0.05）。有趣的是，EI组排卵囊肿的数量为50%。与无囊肿组相比，有囊肿奶牛的卵巢反应和胚胎质量较差（P < 0.05）。总之，替代方案降低了泰国-荷斯坦杂交奶牛的超排卵反应，并增加了胚胎质量差的情况。此外，EI组卵巢卵泡囊肿的发生率更高。
