Grunewald J, Janson C H, Wigzell H
Department of Immunology, Karolinska Institute, Stockholm, Sweden.
Eur J Immunol. 1991 Mar;21(3):819-22. doi: 10.1002/eji.1830210342.
The expression of seven different alpha and beta gene segments of the T cell receptor on normal human CD4+ and CD8+ T lymphocytes, respectively, was examined using V gene-specific monoclonal antibodies. We found a statistically significant (p less than 0.001) bias of the expression of four V gene products towards the CD4+ subpopulation. In every individual analyzed, the V beta 5.1 gene segment was expressed to a higher degree among CD4+ compared to CD8+ cells, with a median value of 4.8% among CD4+ cells and 1.5% among CD8+ cells. There was also a statistically significant skewness in the usage of the V beta 6.7, V beta 8 and V beta 12 gene segments towards the CD4+ T cell population, but not as dominating as for the V beta 5.1. Lymphocytes from umbilical cord blood showed similar skewed reactivities for the V beta 5.1, V beta 6.7 and V beta 12-specific monoclonal antibodies. Aspects of positive and negative selection, as possible explanations for these findings, are discussed.
使用V基因特异性单克隆抗体，分别检测了正常人CD4⁺和CD8⁺T淋巴细胞上T细胞受体的七个不同α和β基因片段的表达情况。我们发现，四种V基因产物的表达在CD4⁺亚群中存在统计学上显著的偏差（p小于0.001）。在每个被分析的个体中，与CD8⁺细胞相比，Vβ5.1基因片段在CD4⁺细胞中的表达程度更高，CD4⁺细胞中的中位数为4.8%，CD8⁺细胞中的中位数为1.5%。Vβ6.7、Vβ8和Vβ12基因片段在CD4⁺T细胞群体中的使用也存在统计学上显著的偏向性，但不如Vβ5.1那么明显。来自脐带血的淋巴细胞对Vβ5.1、Vβ6.7和Vβ12特异性单克隆抗体表现出相似的偏向性反应。本文讨论了作为这些发现可能解释的阳性和阴性选择方面的问题。
