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CD4⁺和/或CD8⁺胸腺细胞的Ly-6C⁺亚群表达异常的T细胞受体（TCR）-Vβ谱系。Ly-6C⁺胸腺细胞与CD4⁻CD8⁻TCR-αβ⁺胸腺细胞之间存在发育关系的证据。

Expression of an unusual T cell receptor (TCR)-V beta repertoire by Ly-6C+ subpopulations of CD4+ and/or CD8+ thymocytes. Evidence for a developmental relationship between Ly-6C+ thymocytes and CD4-CD8-TCR-alpha beta+ thymocytes.

作者信息

Takahama Y, Sharrow S O, Singer A

机构信息

Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.

出版信息

J Immunol. 1991 Nov 1;147(9):2883-91.

PMID:1680922
Abstract

A novel thymocyte subpopulation expressing an unusual TCR repertoire was identified by high surface expression of the Ly-6C Ag. Ly-6C+ thymocytes were distributed among all four CD4/CD8 thymocyte subsets, and represented a readily identifiable subpopulation within each one. Ly-6C+ thymocytes express TCR-alpha beta, arise late in ontogeny, and appear in the CD4/CD8 developmental pathway after birth in a sequence that resembles that followed by conventional Ly-6C- cells during fetal ontogeny. Most interestingly, adult Ly-6C+ thymocytes express an unusual TCR-V beta repertoire that is identical to that expressed by CD4-CD8-TCR-alpha beta+ thymocytes in its overexpression of TCR-V beta 8 and in its expression of some potentially autoreactive TCR-V beta specificities. This unusual TCR-V beta repertoire was even expressed by Ly-6C+ thymocytes contained within the CD4+ CD8- 'single positive' thymocyte subset. Thus, expression of this unusual TCR-V beta repertoire is not limited to CD4-CD8-thymocytes, and is unlikely to be a consequence of their double negative phenotype. Rather, we think that Ly-6C+TCR-alpha beta+ thymocytes and CD4-CD8-TCR-alpha beta+ are developmentally interrelated, a conclusion supported by several lines of evidence including the selective failure of both Ly-6C+ and CD4-CD8-TCR-alpha beta+ thymocyte subsets to appear in TCR-beta transgenic mice. In contrast, peripheral Ly-6C+ T cells are developmentally distinct from Ly-6C+ thymocytes in that peripheral Ly-6C+ T cells expressed a conventional TCR-V beta repertoire and developed normally in TCR-beta transgenic mice in which Ly-6C+ thymocytes failed to arise. We conclude that: 1) expression of a skewed TCR-V beta repertoire is a characteristic of Ly-6C+TCR-alpha beta+ thymocytes as well as CD4-CD8-TCR-alpha beta+ thymocytes, and is not unique to thymocytes expressing neither CD4 nor CD8 accessory molecules; and 2) Ly-6C+ thymocytes are developmentally linked to CD4-CD8-TCR-alpha beta+ thymocytes, but not to Ly-6C+ peripheral T cells. We suggest that Ly-6C+TCR-alpha beta+ thymocytes are not the developmental precursors of Ly-6C+ peripheral T cells, but rather may be the developmental precursors of CD4-CD8-TCR-alpha beta+ thymocytes.

摘要

通过Ly-6C抗原的高表面表达鉴定出一个表达异常TCR库的新型胸腺细胞亚群。Ly-6C+胸腺细胞分布在所有四个CD4/CD8胸腺细胞亚群中，并且在每个亚群中都代表一个易于识别的亚群。Ly-6C+胸腺细胞表达TCR-αβ，在个体发育后期出现，并且在出生后出现在CD4/CD8发育途径中，其顺序类似于传统Ly-6C-细胞在胎儿个体发育期间遵循的顺序。最有趣的是，成年Ly-6C+胸腺细胞表达一种异常的TCR-Vβ库，该库与CD4-CD8-TCR-αβ+胸腺细胞表达的库相同，其TCR-Vβ8过表达且表达一些潜在的自身反应性TCR-Vβ特异性。这种异常的TCR-Vβ库甚至在CD4+CD8-“单阳性”胸腺细胞亚群中的Ly-6C+胸腺细胞中也有表达。因此，这种异常TCR-Vβ库的表达不限于CD4-CD8-胸腺细胞，并且不太可能是其双阴性表型的结果。相反，我们认为Ly-6C+TCR-αβ+胸腺细胞和CD4-CD8-TCR-αβ+在发育上是相互关联的，这一结论得到了几条证据的支持，包括Ly-6C+和CD4-CD8-TCR-αβ+胸腺细胞亚群在TCR-β转基因小鼠中选择性缺失。相比之下，外周Ly-6C+T细胞在发育上与Ly-6C+胸腺细胞不同，因为外周Ly-6C+T细胞表达传统的TCR-Vβ库，并且在Ly-6C+胸腺细胞未能出现的TCR-β转基因小鼠中正常发育。我们得出以下结论：1）偏向的TCR-Vβ库的表达是Ly-6C+TCR-αβ+胸腺细胞以及CD4-CD8-TCR-αβ+胸腺细胞的特征，并非既不表达CD4也不表达CD8辅助分子的胸腺细胞所特有；2）Ly-6C+胸腺细胞在发育上与CD4-CD8-TCR-αβ+胸腺细胞相关，但与Ly-6C+外周T细胞无关。我们认为Ly-6C+TCR-αβ+胸腺细胞不是Ly-6C+外周T细胞的发育前体，而可能是CD4-CD8-TCR-αβ+胸腺细胞的发育前体。

相似文献

1
Expression of an unusual T cell receptor (TCR)-V beta repertoire by Ly-6C+ subpopulations of CD4+ and/or CD8+ thymocytes. Evidence for a developmental relationship between Ly-6C+ thymocytes and CD4-CD8-TCR-alpha beta+ thymocytes.CD4⁺和/或CD8⁺胸腺细胞的Ly-6C⁺亚群表达异常的T细胞受体（TCR）-Vβ谱系。Ly-6C⁺胸腺细胞与CD4⁻CD8⁻TCR-αβ⁺胸腺细胞之间存在发育关系的证据。
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