Schellander K, Fuhrer F, Brackett B G, Korb H, Schleger W
Institute of Animal Breeding and Genetics Veterinary University of Vienna A-1030 Vienna, Linke Bahngasse 11, Austria.
Theriogenology. 1990 Feb;33(2):477-85. doi: 10.1016/0093-691x(90)90505-n.
Bovine follicular oocytes were matured in TCM 199 supplemented with: (1) fetal calf serum (FCS, 20% v/v), Luteinizing Hormone (LH, 10 ug/ml), and Estradiol-17-beta (E(2), 1 ug/ml) in Experiment 1; (2) 20% cow serum recovered at standing estrus (Experiment 2); or (3) 20% FCS (Experiment 3). Maturation, fertilization, and initial cleavage development were evaluated at 16 and 48 h after in vitro insemination. The proportions of oocytes fertilized after maturation in the presence of added hormones (78.5%, Experiment 1) or estrous serum (71.3%, Experiment 2) were significantly higher (p < 0.01) than after use of FCS alone (39.3%, Experiment 3). Cleavage of zygotes within 48 h post-insemination differed significantly (p < 0.01) between maturation treatments, 27.3%, 75.5% and 6.6% for Experiments 1, 2, and 3, respectively. Results demonstrate a beneficial influence of estrous cow serum, characterized by an elevated concentration of LH, on bovine oocyte maturation in vitro.
牛卵泡卵母细胞在添加了以下成分的TCM 199中成熟：（1）在实验1中添加胎牛血清（FCS，20% v/v）、促黄体生成素（LH，10 μg/ml）和雌二醇-17-β（E₂，1 μg/ml）；（2）在实验2中添加发情期采集的20%牛血清；或（3）在实验3中添加20% FCS。在体外受精后16小时和48小时评估成熟、受精和初始卵裂发育情况。在添加激素（实验1中为78.5%）或发情期血清（实验2中为71.3%）的情况下成熟后受精的卵母细胞比例显著高于单独使用FCS（实验3中为39.3%）（p < 0.01）。受精后48小时内合子的卵裂在成熟处理之间存在显著差异（p < 0.01），实验1、2和3的比例分别为27.3%、75.5%和6.6%。结果表明，以LH浓度升高为特征的发情期牛血清对牛卵母细胞体外成熟具有有益影响。
