Abdel-Wahab Ahmed, Abdel Aziz Rabie L, Helmy Nermeen A, Ibrahim Shawky S
Department of Physiology, Faculty of Veterinary Medicine, Minia University, El-Minia.
Department of Theriogenology, Faculty of Veterinary Medicine.
Porto Biomed J. 2018 Jul 3;3(2):e3. doi: 10.1016/j.pbj.0000000000000003. eCollection 2018 Oct.
This study was conducted to assess the effects of addition of 10% newborn bovine serum (NBS) in the maturation and culture media of bovine system on oocytes maturation and developed morula and blastocyst rates. For this purpose, 10% NBS was added to in vitro maturation (IVM) medium alone (Experiment I), in vitro culture (IVC) (Experiment II), and in IVM + IVC (Experiment III). In vitro bovine oocytes maturation and developmental rates of bovine embryos were then compared with control group where the basal maturation or culture media were supplemented with 10% fetal calf serum (FCS). Results revealed that maturation, cleavage, morula, and blastocyst formation rates of in vitro fertilized embryos were not significantly different in NBS and control groups in the 3 experiments. In conclusion, NBS is an efficient macromolecule in bovine system when added either to IVM or to IVC or to both stages. Thus, NBS could provide cheaper and more available treatment to be used as FCS substitute in the IVC system of bovines.
本研究旨在评估在牛体外成熟和培养液中添加10%新生牛血清（NBS）对卵母细胞成熟以及桑椹胚和囊胚发育率的影响。为此，分别在体外成熟（IVM）培养液中单独添加10% NBS（实验I）、体外培养（IVC）液中添加10% NBS（实验II）以及在IVM + IVC中添加10% NBS（实验III）。然后将体外牛卵母细胞的成熟率和牛胚胎的发育率与在基础成熟或培养液中添加10%胎牛血清（FCS）的对照组进行比较。结果显示，在这3个实验中，NBS组和对照组体外受精胚胎的成熟、卵裂、桑椹胚和囊胚形成率无显著差异。总之，在牛体外成熟或体外培养或两个阶段均添加NBS时，NBS是一种有效的大分子物质。因此，NBS可作为一种更便宜且更易获取的物质，在牛的体外培养系统中替代FCS使用。
