Benet-Rubinat J M, Martinez P, Lepp W A, Egozcue J, Andolz P, Bielsa M A
Institut de Biologia Fonamental V. Villar Palasi, Universitat Autonoma de Barcelona, Spain.
Int J Fertil. 1991 Jan-Feb;36(1):48-56.
The enzyme-linked immunosorbent assay (ELISA) has been adapted for the detection of human anti-sperm antibodies (ASA). The use of peroxidase in the enzyme-anti-immunoglobulin conjugate, together with a nontoxic chromogenic substrate (MBTH-DMAB), renders the assay highly sensitive for detection of sperm surface antigens. This ELISA is highly reproducible, not only intra-assay (94% to 97%) but also interassay (80% to 96%). A great advantage of this ELISA is that microplates can be kept at -20 degrees C for months without any decrease in the response. New Zealand white rabbits showed a significant response to human sperm and tail fractions obtained by sonication. The sperm were extremely immunogenic, reaching titers of antisera of 1/10,000 or more. The results show that this ELISA is a good tool for the objective measurement of the anti-sperm response, and is significantly more sensitive than other techniques for anti-sperm antibody analysis.
酶联免疫吸附测定（ELISA）已被用于检测人抗精子抗体（ASA）。在酶抗免疫球蛋白结合物中使用过氧化物酶，再加上一种无毒的显色底物（MBTH-DMAB），使得该测定法对精子表面抗原的检测具有高度敏感性。这种ELISA具有高度可重复性，不仅在批内（94%至97%），而且在批间（80%至96%）。这种ELISA的一个很大优点是微孔板可以在-20摄氏度下保存数月，而反应不会有任何下降。新西兰白兔对通过超声处理获得的人精子和尾部片段有显著反应。精子具有极强的免疫原性，抗血清效价可达1/10000或更高。结果表明，这种ELISA是客观测量抗精子反应的良好工具，并且比其他抗精子抗体分析技术明显更敏感。
