Marinculic A, Gamble H R, Zarlenga D S, Rapic D, Kozaric Z, Imamovic V, Murrell K D
Veterinary Faculty, University of Zagreb, Yugoslavia.
J Parasitol. 1991 Apr;77(2):224-30.
An isolate of Trichinella obtained from a wild boar in Yugoslavia did not form cysts in the musculature of its natural host. Subsequent inoculation into experimental hosts demonstrated that some larvae became encysted only after extended time periods, whereas others remained unencapsulated. Histological staining of larvae in the musculature demonstrated no deposition of collagen typically seen for Trichinella spiralis spiralis, Trichinella spiralis nativa, or Trichinella spiralis nelsoni. The Yugoslavian isolate, given the name of Zagreb isolate after the University where it was first studied, had low infectivity for pigs and mice. Isozyme analysis demonstrated greater homology with T. s. nelsoni than with other subspecies of Trichinella. Restriction fragment length polymorphisms and dot blot analyses further demonstrated the distinctive nature of this isolate. These results suggest that lack of cyst formation might be characteristic of isolates other than those designated Trichinella pseudospiralis and that this character might be important in the classification of Trichinella.
从南斯拉夫一头野猪身上分离出的旋毛虫在其自然宿主的肌肉组织中未形成包囊。随后接种到实验宿主身上表明，一些幼虫仅在较长时间后才形成包囊，而另一些则仍未被包囊化。肌肉组织中幼虫的组织学染色显示，未见到旋毛形线虫、北方旋毛形线虫或纳氏旋毛形线虫通常所见的胶原蛋白沉积。这种南斯拉夫分离株在首次研究它的大学所在城市萨格勒布之后被命名为萨格勒布分离株，对猪和小鼠的感染性较低。同工酶分析表明，它与纳氏旋毛形线虫的同源性高于旋毛虫的其他亚种。限制性片段长度多态性分析和斑点印迹分析进一步证明了该分离株的独特性质。这些结果表明，除了伪旋毛虫之外，缺乏包囊形成可能是其他分离株的特征，并且这一特征在旋毛虫的分类中可能很重要。
