Minchella D J, Eddings A R, Neel S T
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-1392.
J Parasitol. 1994 Oct;80(5):696-704.
Two restriction endonucleases (Cla I and Hpa II) produced polymorphic repetitive DNA profiles which were used in a clustering analysis to quantify the level of genetic variation among 14 North American sylvatic isolates of T5 Trichinella. Differences in genetic profiles reflected phenotypic differences in parasite reproductive success as measured by an isolate's reproductive capacity index in natural hosts. Two genetically distinct isolates of the T5 genotype and T. spiralis were used to infect white-footed mice Peromyscus leucopus for a behavioral analysis. Three behavioral characteristics were tabulated from videotapes: time to emerge from cage, time spent inside cage, and number of rears (index of exploratory activity). Mice infected with 400 T. spiralis larvae showed a significant decrease in exploratory activity and significant increases in time to emerge and time spent inside cage, whereas mice infected with 200 larvae displayed no differences in behavior. Mice infected with a T5 isolate from a raccoon (R9) also displayed decreased exploratory activity. In contrast, mice infected with the other T5 isolate from a coyote (C26) showed relatively constant rearing levels but spent less time inside their cages during the late period of the infection. Results suggest that different genetic strains of Trichinella induce varied types of behavioral modifications upon their hosts.
两种限制性内切酶（Cla I和Hpa II）产生了多态性重复DNA图谱，这些图谱被用于聚类分析，以量化14株北美野生旋毛虫T5分离株之间的遗传变异水平。遗传图谱的差异反映了寄生虫繁殖成功率的表型差异，这种差异通过分离株在自然宿主中的繁殖能力指数来衡量。使用T5基因型和旋毛虫的两个遗传上不同的分离株感染白足鼠（白足鼠属）进行行为分析。从录像带中列出了三个行为特征：从笼子里出来的时间、在笼子里停留的时间和直立次数（探索活动指数）。感染400条旋毛虫幼虫的小鼠探索活动显著减少，从笼子里出来的时间和在笼子里停留的时间显著增加，而感染200条幼虫的小鼠行为没有差异。感染浣熊来源的T5分离株（R9）的小鼠探索活动也减少。相比之下，感染郊狼来源的另一个T5分离株（C26）的小鼠在感染后期直立水平相对稳定，但在笼子里停留的时间较少。结果表明，旋毛虫的不同遗传菌株对其宿主会引起不同类型的行为改变。
