Risco C A, Chenoweth P J, Larsen R E, Velez J, Shaw N, Tran T, Chase C C
Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610, USA.
Theriogenology. 1993 Sep;40(3):629-42. doi: 10.1016/0093-691x(93)90415-2.
The effects of a cottonseed meal diet containing high levels of free gossypol on hematological traits, including erythrocyte osmotic fragility and semen characteristics, were examined during an 11-week period. Eight Brahman bulls were randomly assigned to 1 of 2 groups. The control group (n=4) was fed a mixture of soybean meal and corn. The treated group (n=4) was fed a mixture of cottonseed meal and corn. Both groups were allowed hay free choice. The treated group consumed 8.2 g of free gossypol per bull per day. The percentage of normal spermatozoa was lower (P<0.01) in the treated than in the control group from Week 5 (49+/-9.8 vs 83+/-3.2%), which was primarily influenced by changes in mid piece morphology in the treated bulls. Erythrocyte osmotic fragility was higher (P<0.001) in the treated than in the control group over the entire study period, although group values diverged more acutely from Week 7 of treatment. Sperm motility was lower (P=0.04) in treated bulls than in control bulls at Week 9 (52+/-9.8 vs 82+/-6.2%). These data suggest that concurrent discernable changes in bull erythrocyte osmotic fragility and in semen characteristics can occur following commencement of a diet containing 8.2g of free gossypol per day.
在为期11周的时间里，研究了含有高水平游离棉酚的棉籽粕日粮对血液学特征（包括红细胞渗透脆性）和精液特性的影响。8头婆罗门公牛被随机分为2组中的1组。对照组（n = 4）饲喂豆粕和玉米的混合物。处理组（n = 4）饲喂棉籽粕和玉米的混合物。两组均可自由采食干草。处理组每头公牛每天摄入8.2克游离棉酚。从第5周起，处理组正常精子的百分比低于对照组（P<0.01）（49±9.8%对83±3.2%），这主要受处理组公牛中段形态变化的影响。在整个研究期间，处理组的红细胞渗透脆性高于对照组（P<0.001），尽管从处理第7周起两组数值差异更为明显。在第9周时，处理组公牛的精子活力低于对照组公牛（P = 0.04）（52±9.8%对82±6.2%）。这些数据表明，在开始每天含有8.2克游离棉酚的日粮后，公牛的红细胞渗透脆性和精液特性可能会同时出现明显变化。
