Chavatte P M, Archbald L F, Risco C, Tran T, Sumrall D
Large Animal Clinical Sciences, College of Veterinary Medicine University of Florida, Gainesville, FL 32610 USA.
Theriogenology. 1993 Oct;40(4):745-55. doi: 10.1016/0093-691x(93)90210-v.
This study was conducted to evaluate the use of prostaglandin F2alpha (PGF2alpha) in the initial treatment of ovarian cysts in dairy cattle. Two hundred and sixty three cows diagnosed cystic on palpation per rectum were randomly assigned to one of three treatment groups (A, B or C). Cows in Groups A and B were treated with 25 mg i.m.of PGF2alpha at the time of diagnosis (Day 0), while cows in Group C received 100 mug of GnRH. Seven days following initial treatment (Day 7), cows from Group A that were not observed in estrus were treated with GnRH. Cows from Groups B and C were not treated. On Day 14, all cows that had not been inseminated received PGF2alpha. A blood sample was obtained from all cows on Days 0, 7 and 14 and was analyzed for progesterone (P4) using radioimmunoassay. Incidences of estrus were recorded and cows that were more than 60 d in milk at the time of diagnosis were bred when observed in estrus. The incidence of follicular cysts on Day 0 (as defined as P4 <0.5 ng/ml) was similar between groups and constituted about 40% of all cysts. There were significantly more cows pregnant to insemination within 7 d of initial treatment in Group B than in Groups A and C (P<0.05). After Day 14, the pregnancy rate was not statistically different between Group B and C, but Groups B and C had a statistically higher pregnancy rate than Group A from Day 21 to Day 35. At the end of the study, there was no statistical difference for the pregnancy rate between groups. We concluded that treatment of ovarian cysts diagnosed by per rectum examination with prostaglandin (at time of diagnosis and 14 d later for cows that were not inseminated) was as effective as initial treatment with GnRH followed by prostaglandins 14 d later for cows that were not inseminated previously. Cows that were initially treated with prostaglandins also tended to become pregnant sooner.
本研究旨在评估前列腺素F2α（PGF2α）在奶牛卵巢囊肿初始治疗中的应用。通过直肠触诊诊断为囊肿的263头奶牛被随机分配到三个治疗组（A、B或C）之一。A组和B组的奶牛在诊断时（第0天）肌肉注射25mg PGF2α，而C组的奶牛接受100μg促性腺激素释放激素（GnRH）。初始治疗7天后（第7天），未观察到发情的A组奶牛用GnRH治疗。B组和C组的奶牛未接受治疗。在第14天，所有未进行人工授精的奶牛都接受了PGF2α治疗。在第0、7和14天从所有奶牛采集血样，并使用放射免疫分析法分析孕酮（P4）。记录发情发生率，诊断时产奶超过60天的奶牛在观察到发情时进行配种。第0天卵泡囊肿的发生率（定义为P4<0.5ng/ml）在各组之间相似，约占所有囊肿的40%。B组在初始治疗后7天内人工授精受孕的奶牛显著多于A组和C组（P<0.05）。第14天后，B组和C组的妊娠率在统计学上没有差异，但从第21天到第35天，B组和C组的妊娠率在统计学上高于A组。在研究结束时，各组之间的妊娠率没有统计学差异。我们得出结论，对于通过直肠检查诊断的卵巢囊肿，用前列腺素治疗（诊断时以及14天后对未进行人工授精的奶牛）与先用GnRH治疗、14天后对之前未进行人工授精的奶牛用前列腺素治疗一样有效。最初用前列腺素治疗的奶牛也往往更早怀孕。
