Figueiredo J R, Hulshof S C, Van den Hurk R, Nusgens B, Bevers M M, Ectors F J, Beckers J F
Department of Animal Endocrinology and Reproduction Faculty of Veterinary Medicine University of Liège, B-4000 Liège, Belgium.
Theriogenology. 1994;41(6):1333-46. doi: 10.1016/0093-691x(94)90492-2.
Described in the present paper is a culture system that preserves oocyte and granulosa cell morphology in bovine preantral follicles during 5 d in vitro. The effects of additional hypoxanthine and energy substrata (i.e., pyruvate and glutamine) on the morphology of cultured preantral follicles were investigated. It was shown that addition of a mixture of pyruvate, glutamine and hypoxantine to the culture medium increased the percentage of follicles with an intact oocyte from 29.4 to 78.6%. Morphological criteria are described to discriminate between normal and degenerated preantral follicles during culture by inverted microscopy. In addition, the importance of histological evaluation to judge the quality of oocyte and granulosa cells is demonstrated.
本文描述了一种能在体外5天内保持牛腔前卵泡中卵母细胞和颗粒细胞形态的培养系统。研究了添加次黄嘌呤和能量底物（即丙酮酸和谷氨酰胺）对培养的腔前卵泡形态的影响。结果表明，向培养基中添加丙酮酸、谷氨酰胺和次黄嘌呤的混合物可使具有完整卵母细胞的卵泡百分比从29.4%提高到78.6%。描述了通过倒置显微镜在培养过程中区分正常和退化腔前卵泡的形态学标准。此外，还证明了组织学评估对判断卵母细胞和颗粒细胞质量的重要性。
