Akira A, Ohmura H, Uzumcu M, Araki T, Lin Y C
Laboratory of Reproductive Endocrinology Department of Veterinary Physiology and Pharmacology College of Veterinary Medicine The Ohio State University 1900 Coffey Road Columbus, OH 43210-1092 USA.
Theriogenology. 1994;41(7):1489-97. doi: 10.1016/0093-691x(94)90200-3.
The present study investigated whether gossypol inhibited aromatase activity in cultured porcine granulosa cells. Aromatase activity was assayed by measuring (3)H-H(2)O released from [1beta-(3)H]-androstenedione. First, immature porcine granulosa cells were cultured with various doses of follicle stimulating hormone (FSH, 1 to 1000 ng/ml) for 1 to 5 d to determine optimal culture conditions for aromatase activity assay. Second, porcine granulosa cells were cultured with or without FSH in the presence or absence of gossypol. Gossypol, at 4 muM, significantly inhibited FSH-induced aromatase activity while showing no effect on basal aromatase activity. Gossypol did not inhibit cell proliferation during cell culture. These results suggest that gossypol inhibits aromatase activity by interfering with FSH induction of aromatase in cultured porcine granulosa cells.
本研究调查了棉酚是否抑制培养的猪颗粒细胞中的芳香化酶活性。通过测量从[1β-(3)H]-雄烯二酮释放的(3)H-H(2)O来测定芳香化酶活性。首先，将未成熟的猪颗粒细胞用不同剂量的促卵泡激素（FSH，1至1000 ng/ml）培养1至5天，以确定用于芳香化酶活性测定的最佳培养条件。其次，在有或没有棉酚的情况下，将猪颗粒细胞在有或没有FSH的条件下培养。4 μM的棉酚显著抑制FSH诱导的芳香化酶活性，而对基础芳香化酶活性无影响。棉酚在细胞培养过程中不抑制细胞增殖。这些结果表明，棉酚通过干扰培养的猪颗粒细胞中FSH对芳香化酶的诱导来抑制芳香化酶活性。
