Chian R C, Niwa K, Sirard M A
Département des Sciences Animales, Université Laval Québec, Canada G1K 7P4.
Theriogenology. 1994;41(7):1499-508. doi: 10.1016/0093-691x(94)90201-s.
Bovine oocytes were matured in culture with and without cumulus cells. The proportion of oocytes matured to metaphase-II 24 h after culture was not different between those matured with and without cumulus cells. When cultured oocytes were inseminated in vitro, high proportions (84 to 87%) of oocytes were penetrated, with no difference between those matured with and without cumulus cells. However, the proportion of oocytes penetrated at the male pronuclear stage was significantly higher in cumulus-intact than in cumulus-free oocytes (90 vs 31%). The proportion of oocytes cleaved beyond the 8 approximately 32-cell stage was also significantly higher in cumulus-intact than in cumulus-free oocytes. It is concluded that the cumulus cells may have an important function for normal cytoplasmic maturation of bovine oocytes in vitro.
牛卵母细胞在有或无卵丘细胞的情况下进行体外成熟培养。培养24小时后，成熟至中期II的卵母细胞比例在有卵丘细胞和无卵丘细胞培养的卵母细胞之间没有差异。当对培养的卵母细胞进行体外受精时，高比例（84%至87%）的卵母细胞被穿透，有卵丘细胞和无卵丘细胞培养的卵母细胞之间没有差异。然而，在雄原核阶段被穿透的卵母细胞比例在有完整卵丘细胞的卵母细胞中显著高于无卵丘细胞的卵母细胞（90%对31%）。卵丘完整的卵母细胞中分裂超过8至32细胞阶段的比例也显著高于无卵丘细胞的卵母细胞。结论是，卵丘细胞可能对牛卵母细胞体外正常的细胞质成熟具有重要作用。
