Rodríguez-Gil J E, Montserrat A, Rigau T
Unit of Reproduction, Department of Animal Pathology and Production, School of Veterinary Medicine, Autonomous University of Barcelona, Bellaterra, Spain.
Theriogenology. 1994 Oct;42(5):815-29. doi: 10.1016/0093-691x(94)90450-w.
Hypoosmotic tests are widely used as valuable tests for determining sperm quality in species as varied as the human and the porcine. However, there is little information about the use of these tests in canine spermatozoa. This work evaluates the response of canine spermatozoa in hypoosmotic media in order to introduce the use of the hypoosmotic tests in the canine standard semen analysis. In this way, the incubation of canine spermatozoa in hypoosmotic media containing citrate (ORT medium, osmotic pressure = 100 mOsm) or citrate plus fructose (HOS medium, osmotic pressure = 150 mOsm) resulted in the swelling of the sperm tail. These reactions were time-dependent, reaching maximum percentages after 45 to 60 min. Optimal percentage of tail swelling with minimal effect on the viability of spermatozoa was observed at 100 to 150 mOsm. Response on sperm viability, tail swelling and acrosome detachment to hypoosmotic tests of both undiluted fresh, and 24 h-stored samples were similar. The percentage of swollen tails after both tests showed a good correlation to viability and to gross and progressive motility but not to concentration. However, acrosome detachment after both hypoosmotic tests did not correlate to any of the studied parameters. Our results indicate that the swelling observed after hypoosmotic shock could be used as a useful test in improving the standard semen analysis in the dog.
低渗试验作为一种有价值的检测方法，被广泛应用于包括人类和猪等多种物种的精子质量测定。然而，关于这些检测方法在犬类精子中的应用信息却很少。本研究旨在评估犬类精子在低渗介质中的反应，以便将低渗试验引入犬类标准精液分析中。具体而言，将犬类精子置于含有柠檬酸盐的低渗介质（ORT培养基，渗透压=100 mOsm）或柠檬酸盐加果糖的低渗介质（HOS培养基，渗透压=150 mOsm）中孵育，结果导致精子尾部肿胀。这些反应具有时间依赖性，在45至60分钟后达到最大百分比。在100至150 mOsm时观察到尾部肿胀的最佳百分比，且对精子活力的影响最小。未稀释的新鲜样本和储存24小时的样本在低渗试验中的精子活力、尾部肿胀和顶体脱离反应相似。两种试验后尾部肿胀的百分比与活力、总活力和渐进性活力均呈良好相关性，但与浓度无关。然而，两种低渗试验后的顶体脱离与任何研究参数均无相关性。我们的结果表明，低渗休克后观察到的肿胀可作为一种有用的检测方法，用于改进犬类的标准精液分析。
